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Screening an alternative flame retardant using biological and transcriptomic endpoints in fish embryos

ABSTRACT: Screening an alternative flame retardant using biological and transcriptomic endpoints in fish embryos

PROVIDER: PRJNA478390 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7449530.fastq.gz	Fastqsanger.gz
	SRR7449540.fastq.gz	Fastqsanger.gz
	SRR7449550.fastq.gz	Fastqsanger.gz
	SRR7449560.fastq.gz	Fastqsanger.gz
	SRR7449570.fastq.gz	Fastqsanger.gz

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Screening an alternative flame retardant using biological and transcriptomic endpoints in fish embryos

Project description:Although alternative Flame Retardant (FR) chemicals are expected to be safer than the legacy FRs they replace, their risks to human health and the environment are often poorly characterized. This study used a small volume, fish embryo system to reveal potential mechanisms of action and diagnostic exposure patterns for TBPH (bis (2-ethylhexyl)-tetrabromophthalate), a component of several widely-used commercial products. By design, environmentally-high exposures of TBPH were applied to sensitive early life stages of an ecologically important test species, Fundulus heteroclitus (Atlantic killifish), with a well-annotated genome. Exposed fish embryos were sampled for transcriptomics or chemical analysis of parent compound and primary metabolite, or observed for development and survival through larval stage. Global transcript profiling, which requires no a priori information, was applied using a large number of embryos (16) per treatment to provide a non-targeted and statistically robust approach to characterize TBPH gene expression patterns. Transcriptomic analysis revealed a dose-response in the expression of genes associated with a surprisingly limited number of biological pathways, but included the aryl hydrocarbon receptor signal transduction pathway, which is known to respond to several toxicologically-important chemical classes. These results suggest that TBPH has a relatively low potential for developmental toxicity (at least in fishes), despite concerns related to its structural similarities to endocrine disrupting chemicals, and does not present a unique gene expression pattern. More broadly, this study advances the usefulness of a biological testing and analysis system that complements current screening methods to characterize chemicals of ecological and human health concern

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Evaluation of chronic sublethal effects effects of the organophosphate flame retardant Tris(2-butoxyethyl)phosphate (TBEP) using Daphni magna transcriptomic response

Project description:The aim of this study was to measure chronic sublethal effects of the flame retardant Tris(2-butoxyethyl)phosphate (TBEP) on gene expression, associated protein activity and life-history endpoints (reproduction, development) in the freshwater crustacean Daphnia magna. We exposed <24h old D. magna neonates for 21 days to culture medium (control) and to 3 sublethal doses of TBEP: 14.7, 147 and 1470 µg/L. Exposure doses were chosen from 48h acute toxicity testing and represent 1/10000, 1/1000 and 1/100 of the LC50, respectively. Each condition (control and the 3 different doses) were performed on 3 independant biological replicates, with 10 individuals each. We used a custom 15000 sequences D. magna microarray to measure transcriptional effect of each of the TBEP doses in comparison to unexposed controls.

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Evaluation of chronic sublethal effects effects of the organophosphate flame retardant Tris(2-butoxyethyl)phosphate (TBEP) using Daphni magna transcriptomic response

Project description:The aim of this study was to measure chronic sublethal effects of the flame retardant Tris(2-butoxyethyl)phosphate (TBEP) on gene expression, associated protein activity and life-history endpoints (reproduction, development) in the freshwater crustacean Daphnia magna. We exposed <24h old D. magna neonates for 21 days to culture medium (control) and to 3 sublethal doses of TBEP: 14.7, 147 and 1470 M-BM-5g/L. Exposure doses were chosen from 48h acute toxicity testing and represent 1/10000, 1/1000 and 1/100 of the LC50, respectively. Each condition (control and the 3 different doses) were performed on 3 independant biological replicates, with 10 individuals each. We used a custom 15000 sequences D. magna microarray to measure transcriptional effect of each of the TBEP doses in comparison to unexposed controls. We exposed <24h old Daphnia magna to culture medium (unexposed controls) and 3 sublethal doses of TBEP (14.7M-BM-5g/L, 147M-BM-5g/L and 1470M-BM-5g/L) for 21 days. We used 3 biological replicates of 10 individuals for each condition.

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2018-01-12 | GSE109072 | GEO

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