Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene activation precedes DNA demethylation in response to infection in human dendritic cells

ABSTRACT: Gene activation precedes DNA demethylation in response to infection in human dendritic cells

PROVIDER: PRJNA478394 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7450280.fastq.gz	Fastqsanger.gz
	SRR7450290.fastq.gz	Fastqsanger.gz
	SRR7450300.fastq.gz	Fastqsanger.gz
	SRR7450310.fastq.gz	Fastqsanger.gz
	SRR7450320.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 51

Similar Datasets

Gene activation precedes DNA demethylation in response to infection in human dendritic cells

Project description:Gene activation precedes DNA demethylation in response to infection in human dendritic cells

| PRJNA478412 | ENA

Gene activation precedes DNA demethylation in response to infection in human dendritic cells

Project description:Gene activation precedes DNA demethylation in response to infection in human dendritic cells

| PRJNA478428 | ENA

Gene activation precedes DNA demethylation in response to infection in human dendritic cells

Project description:Gene activation precedes DNA demethylation in response to infection in human dendritic cells

| PRJNA478385 | ENA

Gene activation precedes DNA demethylation in response to infection in human dendritic cells

Project description:Gene activation precedes DNA demethylation in response to infection in human dendritic cells

| PRJNA478422 | ENA

Gene activation precedes DNA demethylation in response to infection in human dendritic cells

Project description:DNA methylation is considered to be a relatively stable epigenetic mark. Yet, a growing body of evidence indicates that DNA methylation levels can change rapidly, for example, in innate immune cells facing an infectious agent. Nevertheless, the causal relationship between changes in DNA methylation and gene expression during infection remains to be elucidated. Here, we generated time-course data on DNA methylation, gene expression, and chromatin accessibility patterns during infection of human dendritic cells with Mycobacterium tuberculosis. We found that the immune response to infection is accompanied by active demethylation of thousands of CpG sites overlapping distal enhancer elements. However, virtually all changes in gene expression in response to infection occur prior to detectable changes in DNA methylation, indicating that the observed losses in methylation are a downstream consequence of transcriptional activation. Footprinting analysis revealed that immune-related transcription factors (TF), such as NF-κB/Rel, are recruited to enhancer elements prior to the observed losses in methylation, suggesting that DNA demethylation is mediated by TF binding to cis-acting elements. Collectively, our results show that DNA demethylation is not necessarily required for the establishment of the core regulatory program engaged upon infection.

2018-07-03 | GSE116411 | GEO

Gene activation precedes DNA demethylation in response to infection in human dendritic cells

2018-07-03 | GSE116406 | GEO

Gene activation precedes DNA demethylation in response to infection in human dendritic cells

2018-07-03 | GSE116405 | GEO

Gene activation precedes DNA demethylation in response to infection in human dendritic cells

2018-07-03 | GSE116399 | GEO

Gene activation precedes DNA demethylation in response to infection in human dendritic cells.

Project description:DNA methylation is considered to be a relatively stable epigenetic mark. However, a growing body of evidence indicates that DNA methylation levels can change rapidly; for example, in innate immune cells facing an infectious agent. Nevertheless, the causal relationship between changes in DNA methylation and gene expression during infection remains to be elucidated. Here, we generated time-course data on DNA methylation, gene expression, and chromatin accessibility patterns during infection of human dendritic cells with Mycobacterium tuberculosis We found that the immune response to infection is accompanied by active demethylation of thousands of CpG sites overlapping distal enhancer elements. However, virtually all changes in gene expression in response to infection occur before detectable changes in DNA methylation, indicating that the observed losses in methylation are a downstream consequence of transcriptional activation. Footprinting analysis revealed that immune-related transcription factors (TFs), such as NF-κB/Rel, are recruited to enhancer elements before the observed losses in methylation, suggesting that DNA demethylation is mediated by TF binding to cis-acting elements. Collectively, our results show that DNA demethylation plays a limited role to the establishment of the core regulatory program engaged upon infection.

| S-EPMC6452747 | biostudies-literature

Gene activation precedes DNA demethylation in response to infection in human dendritic cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-07-03 | GSE116412 | GEO