Project description:We collected cells at three key stages of human iPSC-derived cardiomyocytes (day 0, day 8, and day 15), and performed proteomic and ubiquitinomic analysis.
Project description:To investigate gene regulatory dynamics of normal endothelial cell (EC) differentiation at high resolution, an in vitro differentiation system was employed to derive functional vascular ECs from human embryonic stem cells. Human embryonic stem cells, mesodermal cells, and differentiating cells following VEGF-A stimulation at eight time points (1–48 hours), along with internal controls without VEGF-A treatment (referred to as non-ECs), were collected to profile global RNA expression and H3K27ac signals. In addition, in situ Hi-C data were generated for ECs and non-ECs at 24 hours after VEGF-A treatment to characterize global chromatin contact profiles associated with the EC progenitor state.
Project description:We generated maps of H3K4me1, H3K27ac (enhancers), H3K4me3, Pol II (promoters) and H3K27me3 (repressed chromatin) in the genome of human iPSC-derived cardiomyocytes Differentiation of cardiomyocytes from iPSC followed by ChIP-seq of H3K27ac, H34me1, H327me3, H3K4me3 and PolII
Project description:Currently, cardiomyocytes differentiated from human induced pluripotent stem cells (iPSCs) are routinely generated for disease research and drug development as an alternative to animal models. Although iPSC-derived cardiomyocytes (iPSC-CMs) are generally assumed to resemble myocytes in the fetal heart, a systematic global comparison is still lacking. We established a robust differentiation protocol to generate mature cardiomyocytes from male and female iPSC lines, and investigated their gene expression and splicing profiles, compared to that of human hearts at different stages of development.