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Regulatory non-coding sRNA in response to cyanide in Pseudomonas pseudoalcaligenes CECT5344

ABSTRACT: Regulatory non-coding sRNA in response to cyanide in Pseudomonas pseudoalcaligenes CECT5344

PROVIDER: PRJNA481962 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7541430.fastq.gz	Fastqsanger.gz
	SRR7541431.fastq.gz	Fastqsanger.gz
	SRR7541432.fastq.gz	Fastqsanger.gz
	SRR7541433.fastq.gz	Fastqsanger.gz
	SRR7541425.fastq.gz	Fastqsanger.gz

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Similar Datasets

Regulatory non-coding sRNA in response to cyanide in Pseudomonas pseudoalcaligenes CECT5344

Project description:Biological treatments to degrade cyanide are a powerful technology for cyanide removal from industrial wastewaters. It has been previously demonstrated that the alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 is able to use free cyanide and several metal-cyanide complexes as the sole nitrogen source. In this work, the strain CECT5344 has been used for detoxification of the different chemical forms of cyanide that are present in alkaline wastewaters from the jewelry industry. This liquid residue also contains large concentrations of metals like iron, copper and zinc, making this wastewater even more toxic. To elucidate the molecular mechanisms involved in the bioremediation process, a quantitative sRNA sequencing analysis has been carried out in P. pseudoalcaligenes CECT5344 cells grown with the jewelry residue, free cyanide and ammonium as sole nitrogen sources.

2019-02-13 | GSE117374 | GEO

Pseudomonas pseudoalcaligenes CECT5344 response to cyanide vs. ammonium or nitrogen starvation

2016-01-27 | GSE69930 | GEO

Pseudomonas pseudoalcaligenes CECT5344 response to cyanide vs. ammonium or nitrogen starvation

Project description:Transcriptional analysis of the cyanotroph Pseudomonas pseudoalcaligenes CECT5344 in response to cyanide present in wastewaters from industrial activities, such as jewelry and electroplating activities Four-conditions experiment, including three different nitrogen sources (ammonium, sodium cyanide or a cyanide-containing wastewater). One experiment without nitrogen added to the media (nitrogen limited condition). Four biological replicates for each condition (added nitrogen source to the media) plus the experiment without nitrogen source added.

2016-01-27 | E-GEOD-69930 | biostudies-arrayexpress

Quantitative proteomic analysis (LC-MS/MS) of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-containing wastewaters

Project description:Biological treatments to degrade cyanide have shown to be a powerful technology for cyanide removal from industrial wastewaters. It has been previously demonstrated that the alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 is able to use free cyanide and several metal−cyanide complexes as the sole nitrogen source. The strain CECT5344 has been used for detoxification of the different chemical forms of cyanide that are present in alkaline wastewaters from the electroplating process of the jewelry industry. This liquid residue also contains large concentrations of metals like iron, copper and zinc, making this wastewater even more toxic. A proteomic analysis by LC-MS/MS has been applied to elucidate the molecular mechanisms involved in this bioremediation process in P. pseudoalcaligenes CECT5344. Among others, different proteins related to cyanide and cyanate assimilation, as well as other proteins involved in transport and resistance to metals were induced by the cyanide-containing jewelry residue have been identified.

2017-03-08 | PXD005056 | Pride

Cyanoalanine in Pseudomonas pseudoalcaligenes CECT5344

Project description:Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 to compare the nitrogen metabolism when ammonium, beta-cyanoalanine and jewelry residue (with both free and metal-complexed cyanide) are used as sole nitrogen source

2022-02-17 | PXD026640 | Pride

Ectopseudomonas oleovorans CECT 5344

Project description:Pseudomonas pseudoalcaligenes CECT5344 response to cyanide vs. ammonium or nitrogen starvation

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Pseudomonas pseudoalcaligenes CECT5344

Project description:Draft whole genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344

| PRJEB27 | ENA

Project description:Pseudomonas pseudoalcaligenes CECT5344 Transcriptomics

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Genome sequence of Pseudomonas pseudoalcaligenes CECT5344

Project description:Genome sequence of Pseudomonas pseudoalcaligenes CECT5344

| PRJEB5292 | ENA

non-coding RNA-Seq of Listeria monocytogenes from bacteria growing extracellularly and in macrophages

Project description:Small non-coding RNAs (sRNAs) are widespread effectors of post-transcriptional gene regulation in bacteria. Currently extensive information exists on the sRNAs of Listeria monocytogenes (Lm) expressed during growth in extracellular environments. We used deep sequencing of cDNAs obtained from fractioned RNA (<500nt) isolated from extracellularly growing bacteria and from Lm-infected macrophages to catalog the sRNA repertoire during intracellular bacterial growth. Here we report on the discovery of 150 regulatory RNAs of which 71 have never been previously described. A total of 29 regulatory RNAs, including small non-coding antisense RNAs, are specifically expressed intracellularly. We validated highly expressed sRNAs by Northern blotting and demonstrated by the construction and characterization of isogenic mutants of rli31, rli33-1 and rli50 for intracellular expressed sRNA candidates, that their expression is required for efficient growth of bacteria in macrophages. All three mutants were attenuated when assessed for growth in mouse and insect models of infection. Comparative genomic analysis revealed the presence of lineage specific sRNAs and the absence of sRNA loci in genomes of naturally-occurring infection-attenuated bacteria, with additional loss in non-pathogenic listerial genomes. Our analyses reveal extensive sRNA expression as an important feature of bacterial regulation during intracellular growth.

2011-07-21 | E-MTAB-329 | biostudies-arrayexpress

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