Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Project description:The complete chloroplast genome sequence of Acorus gramineus was assembled and characterized as a resource for future genetic studies. With a total length of 152,887 bp, the chloroplast genome comprised of a large single-copy (LSC) region of 83,005 bp, a small single-copy (SSC) region of 18,230 bp, and two inverted repeat (IR) regions of 25,826 bp. The overall GC contents of the chloroplast genome were 38.7%. A total of 115 genes were predicted, consisting of 80 protein-coding genes, 31 tRNA genes, and 4 rRNA genes. In these genes, nine genes contained one intron and two genes contained two introns. Phylogenetic analysis confirmed the position of A. gramineus within the monocots.
