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SMAD2 binding regions in estrogen receptor-positive breast cancer cell line, T47D

ABSTRACT: SMAD2 binding regions in estrogen receptor-positive breast cancer cell line, T47D

PROVIDER: PRJNA482344 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7550970.fastq.gz	Fastqsanger.gz
	SRR7550971.fastq.gz	Fastqsanger.gz
	SRR7550972.fastq.gz	Fastqsanger.gz
	SRR7550973.fastq.gz	Fastqsanger.gz
	SRR7550969.fastq.gz	Fastqsanger.gz

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SMAD2 binding regions in estrogen receptor-positive breast cancer cell line, T47D

Project description:Inhibitors for cyclin-dependent kinase (CDK) 4 and CDK6 have been established as effective therapeutic options for hormone receptor (HR)-positive, HER2-negative advanced breast cancer. Although the CDK4/6 inhibitors mainly target the cyclin D-CDK4/6-retinoblastoma tumor suppressor protein (RB) axis, little is known about clinical impact of inhibiting phosphorylation of other CDK4/6 target proteins. Here, we have focused on other CDK4/6 targets, SMAD proteins. We showed that a CDK4/6 inhibitor Palbociclib and Activin-SMAD2 signaling cooperatively inhibited cell cycle progression of a luminal-type breast cancer cell line T47D. Mechanistically, Palbociclib enhanced SMAD2 binding to the genome through inhibiting linker phosphorylation of the SMAD2 protein by CDK4/6. Comparison of the SMAD2 ChIP-seq data of T47D with those of a triple-negative breast cancer cell line Hs578T indicated that Palbociclib augments different SMAD2-mediated program defined based on types of cells, and enhances SMAD2 binding to the target regions on the genome without affecting its binding pattern. Collectively, the CDK4/6 inhibitor facilitates the cytostatic effects of Activin-SMAD2, while it also enhances its tumor promoting effects depending on types of breast cancer.

2018-10-23 | GSE117495 | GEO

SMAD2 binding regions in triple negative breast cancer cell line, Hs578T

2018-10-23 | GSE117496 | GEO

SMAD2 binding regions in breast cancer cell line and RNA-seq transcriptome analyses in T47D

Project description:SMAD2 binding regions in breast cancer cell line and RNA-seq transcriptome analyses in T47D

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Nascent RNA sequencing (PRO-seq) after estrogen treatment in T47D cells

Project description:This experiment identifies the primary estrogen response genes in T47D cells.

2021-09-17 | GSE184378 | GEO

Estrogen receptor agonism/antagonism in T47D cells

Project description:Estrogen receptor agonism/antagonism in T47D cells

| PRJNA1055455 | ENA

Smad2/3 binding regions in NMuMG cells

Project description:Smad2/3 binding regions in NMuMG cells

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RNA-seq transcriptome analyses in T47D cells treated with ActA and Palbociclib.

Project description:We performed SMAD2 ChIP-seq analysis in T47D cells with/without Palbociclib treatment. To validate whether the changes in SMAD2 binding to the genome indeed resulted in changes in target gene expression, we performed RNA-seq transcriptome analyses in T47D with/without ActA stimulation and Palbo treatment.

2018-10-23 | GSE117497 | GEO

Smad2/3 binding regions in NMuMG cells

Project description:Smad2/3 binding regions in mouse mammary gland epithelial cells (NMuMG) treated with TGF-beta for 1.5 h were determined by ChIP-seq to evaluate the transcriptional mechanism of TGF-beta-Smad signaling.

2019-08-29 | GSE121254 | GEO

Differential expression levels of mRNAs in tamoxifen-sensitive T47D versus tamoxifen-resistant T47D (T47DR) human breast cancer cells

Project description:Tamoxifen is the most widely used antiestrogen in patients with estrogen receptor (ER) positive breast cancer . However, less than half of patients benefit from tamoxifen treatment and 30-50% acquire resistance and the disease progresses. Resistance to tamoxifen is a serious problem in breast cancer therapy and major efforts are underway to find out underlying mechanisms. To find out the differential expression levels of mRNAs in tamoxifen-sensitive T47D versus tamoxifen-resistant T47D (T47DR) human breast cancer cells, T47DR (tamoxifen-resistant) cell line was established from T47D cells after the following continuous exposure to 1 μmol/L 4-Hydroxytamoxifen (H7904, Sigma, USA) for more than 6 months.Thousands of significantly different mRNA expression levels were found and analysed. Our study provides a reference data for the study of tamoxifen resistance .

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Anti-cancer effects of 7-oxabicyclo[2.2.1]heptene sulfonamide (OBHSA) derivative 17e on gene expression of breast cancer T47D cells

Project description:To investigate the anti-cancer effects of PROTAC-like OBHSA derivative 17e, we exposed estrogen receptor alpha positive (ERα+) breast cancer cell line T47D to compound 17e at 10 μM for 12 hours . We then performed gene expression profiling analysis using data obtained from RNA-seq of T47D cells treated with DMSO or 17e (10 μM) in three replicates.

2024-06-18 | GSE210169 | GEO

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