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ABSTRACT: SMRT sequencing of full-length transcriptome of of Manis javanica

PROVIDER: PRJNA483541 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR7632630_subreads.fastq.gz	Fastqsanger.gz
	SRR7632631_subreads.fastq.gz	Fastqsanger.gz
	SRR7632632_subreads.fastq.gz	Fastqsanger.gz
	SRR7632633_subreads.fastq.gz	Fastqsanger.gz
	SRR7632634_subreads.fastq.gz	Fastqsanger.gz

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Manis javanica

Project description:Manis javanica Transcriptome

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The first large-scale sequencing of M. javanica transcriptomes from salivary glands, liver and small intestine by using Illumina HiSeq technology

Project description:The Malayan pangolin (Manis javanica), an unusual mammal that is a scale-covered, toothless specialist myrmecophage, is maintained primarily through captive breeding in China. Maintaining this species in captivity is a significant challenge partly because little is known about its behavior and reproduction. The molecular mechanisms of its digestive system play a key role in the feeding and dietary husbandry of pangolins in captivity. Here, we performed the first large-scale sequencing of M. javanica transcriptomes from three digestive organs—the salivary glands, liver, and small intestine—by using Illumina HiSeq technology- to provides useful genetic resources for future functional work that may be relevant for the maintenance of captive pangolins.

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Proteomics and microbiotas conjoint analysis in the nasal mucus: revelation of differences in immunological function in Manis javanica and Manis pentadactyla

Project description:This study aimed to investigate the differences in the immunity of Manis javanica and Manis pentadactyla through proteomics and microbiotas conjoint analysis.The nasal mucus of 10 pangolins (weight 5.71 kg ± 0.96 kg) was collected from the Wildlife Rescue Center of Shenzhen (Shenzhen City) and Guangdong Province (Guangzhou City). The pangolins from two different Wildlife Rescue Center were all in a healthy condition, although their feeding diets were not completely consistent.

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2016-03-24 | E-GEOD-70906 | biostudies-arrayexpress

Manis javanica

Project description:Manis javanica overview

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Project description:SMRT sequencing of full-length transcriptome of Cacopsylla chinensis

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Long-read nascent RNA sequencing from S. pombe

Project description:Long-read SMRT cDNA sequencing of nascent RNA from exponentially growing S. pombe cells was employed to obtain transcription elongation and splicing information from single transcripts. Nascent RNA was prepared from the yeast chromatin fraction (Carrillo Oesterreich, Preibisch, Neugebauer, Mol Cell 2010). The nascent 3’ end was labeled with a 3’ DNA adaptor through ligation. The adaptor sequence served as template for full-length reverse transcription and double-stranded cDNA was obtained in a transcriptome-wide PCR. SMRT DNA sequencing libraries were prepared subsequently.

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Project description:Manis javanica Transcriptome or Gene expression

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