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Cell-type specific transcriptome and histone modification dynamics during in situ cellular reprogramming in the Arabidopsis stomatal lineage

ABSTRACT: Cell-type specific transcriptome and histone modification dynamics during in situ cellular reprogramming in the Arabidopsis stomatal lineage

PROVIDER: PRJNA484634 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7646290.fastq.gz	Fastqsanger.gz
	SRR7646300.fastq.gz	Fastqsanger.gz
	SRR7646410.fastq.gz	Fastqsanger.gz
	SRR7646301.fastq.gz	Fastqsanger.gz
	SRR7646411.fastq.gz	Fastqsanger.gz

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Cell-type specific transcriptome and histone modification dynamics during in situ cellular reprogramming in the Arabidopsis stomatal lineage

Project description:This SuperSeries is composed of the SubSeries listed below.

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Molecular profiling of stomatal lineage cell states

Project description:We initiated a study to investigate the transcriptional profiles associated with cell states of the stomatal lineage. A stem-cell like precursor of stomata, a meristemoid. reiterates asymmetric divisions and renews itself before differentiating into guard cells. The transient and asynchronous nature of the meristemoid has made it difficult to study its molecular characteristics. Through combinatorial use of genetic resources that either arrest or constitutively drive stomatal cell-state progressions due to loss- or gain-of-function mutations in the key transcription factor genes, SPEECHLESS, MUTE, and SCRM, we obtained seedlings highly enriched in pavement cells, meristemoids, or stomata. Here we present transcriptome and genome-wide trends in gene regulation associated with each cell state and identify molecular signatures associated with meristemoids. 12 samples are included in this study. Three biological replicates of 5-dag seedlings of speechless, scrm-D and scrm-D;mute were compared wild type seedlings for changes in gene expression.

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