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MiRTrace quality control of small RNA-Seq data prepared from low-input, degraded and contamianted HEK-293T RNA samples

ABSTRACT: miRTrace quality control of small RNA-Seq data prepared from low-input, degraded and contamianted HEK-293T RNA samples

PROVIDER: PRJNA485638 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7687080.fastq.gz	Fastqsanger.gz
	SRR7687081.fastq.gz	Fastqsanger.gz
	SRR7687082.fastq.gz	Fastqsanger.gz
	SRR7687083.fastq.gz	Fastqsanger.gz
	SRR7687084.fastq.gz	Fastqsanger.gz

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miRTrace quality control of small RNA-Seq data prepared from low-input, degraded and contamianted HEK-293T RNA samples

Project description:miRTrace is a tool for quality control and tracing taxonomic origins of microRNA sequencing data. It operates in two modes: Trace mode, in which the software reports the composition of clade-specific miRNAs; and QC mode, in which it performed an all-round quality control. To validate the QC mode of the software, we subjected the in-house control samples from HEK-293T cells to various treatments, such as cross-species contamiantion with Drosophila S2 RNAs, sample dilution and RNase A digestion. These samples were processed using QC mode of miRTrace. We demonstrate that miRTrace accurately identities poor-quality samples and to some extent even the causes of the compromised quality.

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