Project description:Total RNA of KSHV-infected cells were extracted and RT-PCR was performed using viral circRNA specific divergent primers. Amplicons were sequenced with MiSeq.
Project description:Since our data support that Pygo2 function is p53 Wild type status dependent and the interaction of Pygo2 with H3K4-Me3 is needed for the c-Kit regulation by Pygo2, however, neither Pygo2 nor p53 bind at c-Kit promoter region. To find out the potential co-binding locia for Pygo2, p53 and H3K4-Me3, we performed a Cut&Run assay followed by Miseq Sequencing.
Project description:Illumina MiSeq next generation sequencing chip was used to identify differentially expressed miRs by comparing peripheral blood mononuclear cell samples between OSA patients and healthy non-snorers.