Project description:The current risk stratification system defined by clinicopathological features do not accurately identify the risk of disease recurrence in patients with stage I-II colorectal cancer (CRC). We aimed to investigate whether the CpG sites from newly established genome-wide methylation profiles could serve as biomarkers to improve prognosis prediction.
Project description:Abnormal DNA methylation is a hallmark of human cancers and may be a promising biomarker for early diagnosis of human cancers1. However, the majority of DNA methylation biomarkers that have been identified are based on the hypothesis that early differential methylation regions (DMRs) are maintained throughout carcinogenesis and could be detected at all stages of cancer. In this study, we identified potential early biomarkers of colorectal cancer (CRC) development by genome-wide DNA methylation assay (Illumina infinium450, 450K) to normal (N=20) and pre-colorectal cancer samples including 18 low-grade adenoma (LGA) and 22 high-grade adenoma (HGA).
Project description:Early-stage lung cancer offers a critical window for therapeutic intervention, yet the epigenetic alterations underlying disease initiation remain incompletely defined. DNA methylation is a central regulator of gene expression and a well-recognized contributor to tumorigenesis, but methylation profiling in stage I disease has largely relied on array-based platforms with limited genomic coverage. Here we systematically characterized the DNA methylation landscape of stage I lung cancer at base resolution. We first identified differentially methylated regions (DMRs) distinguishing stage I tumors from adjacent normal lung tissue, and compared these with alterations observed at later stages. We then examined the functional consequences of these DMRs by testing their associations with differential gene expression, thereby linking epigenetic alterations to transcriptional dysregulation in early disease. Finally, we mapped regions of coordinated methylation (co-methylation) and assessed their relationship with cancer-associated gene dysregulation, with the aim of identifying epigenetically regulated gene networks that operate from the earliest detectable stage of tumorigenesis.
Project description:Genome wide DNA methylation profiling of tumor and surrounding healthy colonic mucosa from patients with colorectal carcinoma. The Illumina Infinium 27k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across 27,578 CpG loci covering 14,475 genes.
Project description:Genome wide DNA methylation profiling of tumor and surrounding healthy colonic mucosa from patients with colorectal carcinoma. The Illumina Infinium 27k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across 27,578 CpG loci covering 14,475 genes. Samples included a total of 48 paired normal and tumor samples from 24 patients. The paired samples were put on the same chip.