Project description:To further development of our gene expression approch to intracellular pathogenic bacterial controlling, we have employed castamized Neochlamydia S13 genomic microarray as a discovery platform to identy genes with the potential to inhibit Legionella growth into hoat amoebae , based on our data that the the amoebae haboring amoebal endosymbiont Neochlamydia S13 (an environmental chlamydia) could evade Legionella infection.
Project description:Puccinia graminis f. sp. tritici is the cause of wheat stem rust. A microarray was designed from genes predicted from the P. graminis f. sp. tritici genome assembly, and gene expression measured for four conditions which include wheat or barley infecting growth stages initiated by urediniospores. mRNA was prepared from fresh urediniospores, uredinospores germinated for 24 hr, wheat seedlings infected with urediniospores for 8 days, and barley seedlings infected with urediniospores for 8 days. The asexual uredinial infection cycle on wheat produces additional urediniospores, which can start new cycles of wheat infection and are readily spread by aerial transport. This expression data is further described in Duplessis et al, Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici