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Identification of ADAR3-bound targets in U87 cells

ABSTRACT: Identification of ADAR3-bound targets in U87 cells

PROVIDER: PRJNA490661 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7825790_1.fastq.gz	Fastqsanger.gz
	SRR7825790_2.fastq.gz	Fastqsanger.gz
	SRR7825800_1.fastq.gz	Fastqsanger.gz
	SRR7825800_2.fastq.gz	Fastqsanger.gz
	SRR7825791_1.fastq.gz	Fastqsanger.gz

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Identification of ADAR3-bound targets in U87 cells

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Project description:Although R-domain has been implicated in RNA binding in vitro, whether ADAR3 uses the arginine-rich R-domain to mediate ADAR3-RNA interactions in vivo remain to be explored. Here, an unbiased, high-throughput approach was undertaken to understand if mutating the R-domain would affect the ability of ADAR3 to either bind and/or recognize target transcripts. Although R-domain doesn’t confer target specificity to ADAR3, the R-domain mutant is associated with decreased protein stability and might reduce the ability of ADAR3 to bind target RNAs.

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