Scylla serrata isolate:Ss1 | breed:crab
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA494913 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:hybird crab of female scylla serrata and male scylla paramamosain Raw sequence reads
| PRJNA686852 | ENA
Project description:The project aims to reveal the proteomic responses of crab Scylla paramamosain to high and low salinity stress.
2021-09-09 | PXD022815 | Pride
Project description:Genetic variation of the giant mud crab Scylla serrata in relation to metal pollution of Indonesian Mangroves
| PRJEB35677 | ENA
Project description:Scylla paramamosain breed:mud crab Raw sequence reads
| PRJNA380738 | ENA
Project description:Scylla serrata Genome sequencing and assembly
| PRJNA1191912 | ENA
Project description:Comparative transcriptome profiling of heat stress response of the mangrove crab Scylla serrata across different sites
| PRJDB10708 | ENA
Project description:Background: The Scylla paramamosain is a very important aquaculture crustacean species in the southeast coastal areas of China including Shantou. For the past few years, mud crab cultured in Niutianyang of Shantou suffered from serious diseases, especially the bacterial diseases (such as Vibrio parahaemolyticus). In eukaryotes, small RNAs can regulate gene expression in post-transcription to act on host-pathogen interaction system. Aims: V.parahaemolyticus isolated from Shantou Niutianyang crab culture area was injected to S.paramamosains to carry out an essential analysis on global miRNA expression in diverse tissues between two groups by the Illumina Solex deep sequencing technology. Methodology:To examine the relationship between mud crab miRNA expression and the bacterial pathogen, we collected mixed two pools of equal amounts of RNA from 7 different mud crab tissues (mesenteron, heart, liver, gill, brain, muscle and blood) and sequencing by Illumine/Solexa deep sequencing technology under normal conditions and during infection with V.parahaemolyticus. The high throughput sequencing resulted in 19,144,358 and 18,559,070 raw reads corresponding to 17,496,577 and 16,888,096 high-quality mappable reads for the normal and infected mixed pools, respectively. Stem-loop RT-qPCRs were used to confirm the microRNAs expression in different tissues of two pools. The results show that miRNAs might play a key role in regulating gene expression during mud crab S.paramamosain infection with V.parahaemolyticus. Conclusions: We identified a large number of miRNAs during the mud crab Scylla paramamosain infection with V.parahaemolyticus, some of which are differentially expressed between the treatments and the controls. The study provides an opportunity for further understanding of small RNA function in the regulation of molecular response and gives us clues for further studies of the mechanisms of V.parahaemolyticus infection in mud crab. Examination of miRNA expression in normal Scylla paramamosain group and the Scylla paramamosain infected with Vibrio parahaemolyticus
2012-08-07 | E-GEOD-39921 | biostudies-arrayexpress
Project description:We used the BDA DNBelabC4 platform to conduct single-cell RNA transcriptome sequencing on hemocytes before and after infection with WSSV, and to study the changes in hemocytes composition and antiviral mechanism of a crustacean virus infection represented by mud crab. The blood cells, or hemocytes, of the mud crab (Scylla paramamosain) play crucial roles in its innate immune system. These cells are involved in various immune responses, including phagocytosis, encapsulation, and the production of antimicrobial peptides. The hemocytes can be isolated and characterized using techniques such as flow cytometry, which helps in understanding their functions and interactions within the crab's immune system. White Spot Syndrome Virus (WSSV) is a highly contagious and lethal virus that primarily infects crustaceans, particularly shrimp and crabs. It is an enveloped double-stranded DNA virus, typically oval-shaped, measuring about 320nm in length and 100nm in width, making it one of the largest known viruses. WSSV is the causative agent of White Spot Syndrome, a disease that has caused significant economic losses in the aquaculture industry.
2026-01-12 | GSE289112 | GEO