Project description:mRNA degradation critically contributes to liver development and function. The CCR4-NOT complex serves as a major deadenylase that initiates mRNA degradation. We used microarrays to identify deregulated genes in the livers lacking Cnot3, a core subunit of the CCR4-NOT complex.
Project description:Transcriptional profiling of whole kidneys from Six2CreEGFP mice without (WT) or with (KO) homozygously floxed DOT1 alleles at the age of embryonic day 16.5. This experiment aimed to uncover the genome-wide alternation in gene expression resulting from the removal of DOT1 gene in the nephron progenitor population (Six2 positive) and successive changes to the series of events in kidney development.
Project description:Gene expression profiling was performed using the total RNA isolated from pooled esophagi (plural of esophagus) of embryonic day 15.5 (E15.5) C57BL/6 mouse embryos and total RNA isolated from pooled esophagi of postnatal day 2 (P2) C57BL/6 pups. The goal was to identify differentially regulated genes in these two separate developmental stages.
Project description:RNA-seq of Wild-type and Math5-/- mouse dLGN (dorsolateral geniculate nucleus) at postnatal day 3, postnatal day 7, postnatal day 14 and postnatal day 23
Project description:To investigate the transcriptome changes in embryonic and postnatal mouse hearts, we performed gene expression profiling analysis using data obtained from RNA-seq of C57BL/6J mouse hearts at embryonic day 18.5 (E18.5d), postnatal 1st day (P1d) and postnatal 7th day (P7d).
Project description:The gene expression profiles of control vs AGTR2 knockout mouse whole brains at developmental stage E15 and postnatal day 1 were examined. Experiment Overall Design: Embryonic day 15: six biological control replicates and eight biological AGTR2 knockout replicates, one control and knockout replicate to an array with a dye-swap, except for 2 without a dye-swap Experiment Overall Design: Postnatal day 1: four biological control replicates and four biological AGTR2 knockout replicates, one control and knockout replicate to an array with a dye-swap
Project description:The developing brain is particularly sensitive to ethanol during the brain growth spurt or synaptogenesis (third human trimester equivalent). This has been shown to lead to abnormal brain development and behavioural changes in the adult mouse that are relevant to those seen in humans with fetal alcohol spectrum disorders (FASD). We evaluated the acute (4h post-treatment) gene expression changes that occur in the brain due to ethanol exposure during synaptogenesis (postnatal day 7). We used microarray analyses to evaluate the changes in brain gene expression at postnatal day 7 that occur due to ethanol treatment at postnatal day 7 (synaptogenesis).
Project description:The gene expression profiles of control vs AGTR2 knockout mouse whole brains at developmental stage E15 and postnatal day 1 were examined. Keywords: genetic modification, brain development, AGTR2