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Omics score: 0

ABSTRACT: Barcode

PROVIDER: PRJNA494953 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR7975590_1.fastq.gz	Fastqsanger.gz
	SRR7975590_2.fastq.gz	Fastqsanger.gz
	SRR7975600_1.fastq.gz	Fastqsanger.gz
	SRR7975600_2.fastq.gz	Fastqsanger.gz
	SRR7975591_1.fastq.gz	Fastqsanger.gz

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Project description:HCC827 cells were barcoded using the ClonTracer lentiviral barcode library such that the majority of cells were infected with a single barcode. One million cells were expanded to ~120 million cells and split into 8 HYPERfasks. Two HYPERfasks were grown under DMSO and grown until confluence. In six HYPERfasks cells were grown under a GI90 concentration of one of two different inhibitors, gefitinib and trametinib (3 HYPERfasks each). Cells achieved confluence at 4 and 9 weeks for gefitinib and trametinib respectively. During this time, the medium and inhibitor were replenished weekly and DNA was extracted from the medium to track barcode content from dying cells.

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