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ABSTRACT: Whole genome sequencing of Indian white shrimp, Penaeus indicus

PROVIDER: PRJNA495105 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR12969543_1.fastq.gz	Fastqsanger.gz
	SRR12969543_2.fastq.gz	Fastqsanger.gz
	SRR12969544_1.fastq.gz	Fastqsanger.gz
	SRR12969544_2.fastq.gz	Fastqsanger.gz

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Project description:DEVELOPMENT OF INDIGENOUS SHRIMP (INDIAN WHITE SHRIMP) AQUACULTURE: GENETIC IMPROVEMENT PROGRAM OF PENAEUS INDICUS

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Identification of differentially expressed genes against WSSV infection in Penaeus monodon by microarray

Project description:Microarray analysis of the gill tissues of WSSV infected shrimp (P. monodon) at different time intervals 6 hrs, 24 hrs, 48 hrs and moribund stage of post WSSV infection was carried out to identify differentially expressed genes in response to WSSV infection. The shrimps in WSSV challenege experiment were challenged through intra muscular route with known concentration of virus. The important immune genes identified would be further characterized by sequence analysis and gene expression profile would be validated by real time PCR One-color experiment,Organism: Penaeus monodon, Custom Penaeus monodon (Black Tiger Shrimp) 8x60k designed by Genotypic Technology Private Limited (AMADID: 041733), Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)

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Identification of testis-relevant genes using in silico analysis from testes ESTs and cDNA microarray in the black tiger shrimp (Penaeus monodon)

Project description:Comparisons of gene expression profiles between testis and ovary of juvenile and wild brooders were made through a cDNA microarray in the black tiger shrimp (Penaeus monodon). Differentially expressed genes were identified through the microarray analysis, and the microarray results were confirmed by real-time PCR. Selected genes were further characterized.

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A cDNA Microarray, UniShrimpChip, for Identification of Genes Relevant to Testicular Development in the Black Tiger Shrimp (Penaeus monodon)

Project description:Comparisons of gene expression profiles from testis of wild and domesticated male brooders were made through a cDNA microarray in the black tiger shrimp (Penaeus monodon). Differentially expressed genes were identified through the microarray analysis, and the microarray results were confirmed by real-time PCR. Selected genes were further characterized.

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Transcriptomic analysis of polychaete-enhanced testicular maturation in male broodstock of the black tiger shrimp (Penaeus monodon)

Project description:The study aimed to determine effect of polychaetes as a shrimp feed on male reproductive maturation at transcriptional level through a cDNA microarray in the black tiger shrimp (Penaeus monodon). Thus, the experiment was to compare transcriptomic profiles of two different parts of reproductive organs, namely testes (TT) and vas deferens (VD), of domesticated 17-month-old between two different feeds, namely commercial pellet and polychaetes after feeding for one month. Differentially expressed genes were identified through the microarray analysis, and the microarray results were confirmed by real-time PCR. Selected genes were further characterized.

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Identification of fatty acid regulatory genes using in silico analysis from Ovary and Hepatopancreas ESTs and cDNA microarray in the black tiger shrimp (Penaeus monodon)

Project description:Comparisons of gene expression profiles in hepatopancreas and ovary of domesticated broodstock at different ovarian maturation stages were made through a cDNA microarray in the black tiger shrimp (Penaeus monodon). Differentially expressed genes were identified through the microarray analysis, and the microarray results were confirmed by real-time PCR. Selected genes were further characterized.

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Effects of eyestalk ablation on the gene expression levels in female black tiger shrimp broodstock (Penaeus monodon)

Project description:Comparisons of gene expression profiles between ovaries of before (day 0) and after eyestalk ablation (days 1, 4 and 7) of domesticated 14-month-old black tiger shrimp (Penaeus monodon) were made using a cDNA microarray. Differentially expressed genes were identified through the microarray analysis, and the microarray results were confirmed by real-time PCR. Selected genes were further characterized.

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