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Single-cell gene expression of anti-viral WT and Thpok-deficient effector and memory T cells

ABSTRACT: Single-cell gene expression of anti-viral WT and Thpok-deficient effector and memory T cells

PROVIDER: PRJNA495334 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7996790.fastq.gz	Fastqsanger.gz
	SRR7996800.fastq.gz	Fastqsanger.gz
	SRR7996810.fastq.gz	Fastqsanger.gz
	SRR7996820.fastq.gz	Fastqsanger.gz
	SRR7996791.fastq.gz	Fastqsanger.gz

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Gene Expression in Effector T cells, wildtype of Thpok-deficient

Project description:The transcription factor Thpok is essential for CD4 T cell development in the thymus and remains expressed in post-thymic CD4 T cells. We post-thymically inactivated Thpok and compared microarray gene expression in Thpok-deficient CD4 T cells to that in their wildtype CD4 or CD8 counterparts We show that Thpok constrains the transcriptional circuitry to maintain CD4+-lineage integrity in naM-CM-/ve cells and to couple effector differentiation to environmental cues after antigenic stimulation. Redundantly with the related factor LRF, Thpok is continuously needed to prevent the trans-differentiation of mature CD4+ into -CD8+ T cells. We activated naM-CM-/ve CD4 T cells (either wild-type or Thpok-deficient) and CD8 T cells (wild-type) in vitro under Th1 conditions. Differentiated effectors were sorted 4 days after activation into CD4+CD8- and CD4-CD8+ (wild-type) and CD4+CD8- and CD4+CD8+ (Thpok-deficient) subsets. Total RNA was extracted from sorted subsets and processed for microarray analyses (Affymetrix Mouse Exon 1.0 ST array) at the NCI microarray facility, following the manufacturerM-bM-^@M-^Ys recommendation. Data is from 3 replicates (except wild-type CD4-CD8+ cells, for which two samples only were processed), generated from two distinct cell preparations.

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Single-cell gene expression of anti-viral WT and Thpok-deficient effector and memory T cells

Project description:Single-cell gene expression of effector and memory CD4+ and CD8+ T cells from WT or Thppok-deficient animals was determined by sRNAseq after LCMV Armstrong infection

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Project description:We noticed that ThPOK repression is readily abrogated upon in vitro TCR stimulation of peripheral CD8 T cells. This observation prompted us to investigate a role of ThPOK in the CD8 T cell response to an acute viral infection. We observed that clonal expansion is significantly less in both primary and secondary CD8 T cell responses in the absence of functional ThPOK. To approach this mechanism, we carried out a microarray analysis for comparison of gene expression between ThPOKhd/hd and ThPOKwt/wt P14 memory T cells.

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