Project description:Our data showed that ENT broadly impacted multiple populations of immune cells within the TME. Thus, we performed general immune transcriptome profiling on whole tumors isolated from the neu-N model using a PanCancer immune-profiling gene panel for the NanoString platform.
Project description:To examine the effect of PROTEXI vaccination on the immunosuppressive nature of the tumor microenvironment (TME), the transcriptional signature of B16F10 tumors in the PROTEXI group with that of the control and DCTAA-vaccinated groups were compared at day 13. Total RNA was extracted from the tumors and subjected to differential gene expression profiling using the NanoString PanCancer Immune Profiling panel, consisting of 755 genes.
Project description:nCounter platform from Nanostring Technologies (Mouse PanCancer Immune Profiling Panel) was used to assess differential gene expression between NT and Th17 cell treated B16F10 melanoma bearing mice in the tumor and tumor-draining lymph nodes.
Project description:We undertook to determine whether carcinoma cells residing in the epithelial or quasi-mesenchymal phenotypic states differ in their expression of immunomodulatory markers. To do so, we used the nCounter PanCancer Immune Profiling Panel (Nanostring Technologies)to perform transcriptomic analyses of the E and qM mammary carcinoma cells; these cells were either cultured in vitro or prepared by FACS sorting of GFP-labelled carcinoma cells from their corresponding tumors