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Single cell RNA-seq resolves lineage-specific activation dynamics of human blood and tissue T cells

ABSTRACT: Single cell RNA-seq resolves lineage-specific activation dynamics of human blood and tissue T cells

PROVIDER: PRJNA495590 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7993630_1.fastq.gz	Fastqsanger.gz
	SRR7993630_2.fastq.gz	Fastqsanger.gz
	SRR7993640_1.fastq.gz	Fastqsanger.gz
	SRR7993640_2.fastq.gz	Fastqsanger.gz
	SRR7993631_1.fastq.gz	Fastqsanger.gz

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Single cell RNA-seq resolves lineage-specific activation dynamics of human blood and tissue T cells

Project description:Human T cells coordinate adaptive immunity and persist as heterogeneous subsets in tissues and circulation, yet the impact of localization on T cell function remains unclear. Here, we profile activation of T cells from human lungs, lymph nodes (LN), bone marrow (BM) and blood following TCR/CD3 stimulation by single cell RNA-seq. Analysis of >50,000 individual resting and activated T cells using new factorization methods reveals conserved lineage-specific gene expression signatures and activation trajectories across sites. CD4 T cells exhibit a transient intermediate activation state while CD8 T cells show an “all-off/all-on” activation pattern. Lung and LN T cells exhibit tissue-specific profiles, while blood T cells closely resemble those in BM. Our results define the lineage-specific activation dynamics of human T cells from different anatomical sites.

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