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AQP5-Expressing Cells Serve as Stem Cells and Cancer Origins in the Mouse and Human Pyloric Stomach

ABSTRACT: AQP5-Expressing Cells Serve as Stem Cells and Cancer Origins in the Mouse and Human Pyloric Stomach

PROVIDER: PRJNA498506 | ENA |

REPOSITORIES: ENA

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AQP5-Expressing Cells Serve as Stem Cells and Cancer Origins in the Mouse and Human Pyloric Stomach

Project description:Isolating adult stem cells from human stomach epithelia has proven challenging due to a lack of surface markers that are amenable to antibody-based sorting approaches. Here, through comparative expression profiling of mouse Lgr5+ adult stem cells along the GI tract, we identify novel pylorus-specific stem cell markers, including the membrane protein Aqp5. FACS-based isolation of endogenous AQP5+ cells from healthy human pyloric stomach revealed their stem cell identity in organoid assays. Using new Aqp5-driven CreERT2 mouse models to selectively target conditional mutations to the pyloric stem cell compartment in vivo, we establish pyloric stem cells as a major source of Wnt-driven invasive gastric cancer. The new stem cell markers and mouse models described here will be an invaluable resource for deciphering early gastric cancer formation and for isolating and characterizing human stomach stem cells as a prerequisite to potentially exploiting their regenerative medicine potential in the clinic.

2019-12-09 | GSE121803 | GEO

AQP5-Expressing Cells Serve as Stem Cells and Cancer Origins in the Distal Stomach

Project description:Isolating adult stem cells from human stomach epithelia has proven challenging due to a lack of surface markers that are amenable to antibody-based sorting approaches. Here, through comparative expression profiling of mouse Lgr5+ adult stem cells along the gastrointestinal tract, we identify novel pylorus-specific stem cell markers, including the membrane protein Aqp5. FACS-based isolation of endogenous AQP5+ cells from healthy human pyloric stomach revealed their stem cell identity in organoid assays. Using new Aqp5-driven CreERT2 mouse models to selectively target conditional mutations to the pyloric stem cell compartment in vivo, we establish pyloric stem cells as a major source of Wnt-driven invasive gastric cancer. The new stem cell markers and mouse models described here will be an invaluable resource for deciphering early gastric cancer formation and for isolating and characterizing human stomach stem cells as a prerequisite to potentially exploiting their regenerative medicine potential in the clinic.

2019-12-09 | GSE133036 | GEO

Revealing tumour cell heterogeneity in an Aqp5-APK mouse pyloric tumour model

Project description:The cell lineages present within the healthy gastric epithelium have been well delineated. However, tumour cell lineages within the stomach are less understood. To dissect the heterogeneity within pyloric tumours in the mouse stomach, we performed scRNAseq on an Aqp5-APK mouse pyloric tumour to resolve the diversity of cell populations present within these tumours.

2025-09-23 | GSE265920 | GEO

Evaluating Aquaporin-5 (Aqp5) functions in mouse pyloric cancer stem cells

Project description:To study Aqp5 functions in pyloric cancer stem cells, we isolated GFP+ putative stem cells and GFP- tumour epithelial cells from Aqp5 wildtype and Aqp5 knockout pyloric mouse tumours to understand the functions of Aqp5 in these cancer stem cells.

2025-09-23 | GSE265919 | GEO

AQP5-Expressing Cells Serve as Stem Cells and Cancer Origins in the Distal Stomach

Project description:AQP5-Expressing Cells Serve as Stem Cells and Cancer Origins in the Distal Stomach

| PRJNA549866 | ENA

Evaluating Aquaporin-5 (Aqp5) functions in mouse pyloric cancer stem cells

Project description:Evaluating Aquaporin-5 (Aqp5) functions in mouse pyloric cancer stem cells

| PRJNA1104685 | ENA

Active Lgr5 Positive Stem Cells Drive Self-Renewal in the Pyloric Stomach and Efficiently Build Pyloric Units In-vitro

Project description:The Wnt target gene Lgr5 marks cycling stem cells in the small intestine, colon and hair-follicle. In the adult stomach, Lgr5 expression is confined to 3-4 proliferating cells at the gland base throughout the pylorus and limited numbers of corpus-type glands adjacent to the esophagus and squamous forestomach. In-vivo lineage tracing reveals that the pyloric Lgr5+ve cells rapidly generate all the major cell-types present in the gastric epithelium and maintain this multipotent stem cell activity over at least 20 months. In-vitro, single adult Lgr5+ve cells efficiently generate gastric units closely resembling mature pyloric glands. We conclude that Lgr5 is marking an active stem cell population at the base of the pyloric glands contributing to the long-term renewal of the adult gastric epithelium. The transcriptome of the adult Lgr5+ve stem cells harbors multiple Wnt target genes, implying a role for Wnt signaling in regulating pyloric stem cell function in-vivo. Conditional deletion of APC in these Lgr5+ve stem cells rapidly initiates tumor formation, supporting a potential role for aberrant Wnt signaling activity in gastric cancer.

2010-02-01 | GSE17485 | GEO

Revealing tumour cell heterogeneity in an Aqp5-APK mouse pyloric tumour model

Project description:Revealing tumour cell heterogeneity in an Aqp5-APK mouse pyloric tumour model

| PRJNA1104686 | ENA

RNA-seq of stomach and pyloric caeca from Atlantic salmon (Salmo salar) fed diets containing chitin from shrimp and black soldier fly larvae

Project description:Farmed Atlantic salmon was given either a 6 % cellulose diet, a diet containing 6 % shrimp shell chitin or a diet containing 6 % chitin from black soldier fly larvae for a period of 4 weeks. The fish were split into six tanks at the beginning of the experiment; six fish per tank and two tanks per diet. RNA from stomach and pyloric caeca from four fish given each diet was sequenced.

2021-08-15 | E-MTAB-10594 | biostudies-arrayexpress

Understanding Aquaporin-5 (Aqp5) functions in human gastric cancer organoids

Project description:To study Aqp5 functions in human gastric cancer organoids, we generated Aqp5 FLIP-Puro organoids allowing for inducible loss of Aqp5 expression upon Cre gesicle administration. We analysed transcriptomes of these organoids at various timepoints following loss of Aqp5 to elucidate potential functions of Aqp5 in this system.

2025-09-23 | GSE266551 | GEO

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