Project description:The purpose of this study was the identification of RNAs contained in the urinary exosome (UExo) from dogs and cats. The quality of total RNA in isolated urinary exosome (UExo)-derived total RNAs obtained from the column-based method (urine 1 mL) was checked by using a Bioanalyzer, and samples from normal renal function (NR) group and kidney disease (KD) group were pooled as one sample for each group. We collected NR dogs (n = 37), KD dogs (n = 47), NR cats (n=43), and KD cats (n = 45). For the next generation sequencing, libraries were prepared according to the manufacturer’s protocols and sequenced using 50-base reads acquired by using a HiSeq 2000 platform. The December 2011 (GRCm38/mm10) mouse (Mus musculus) genome data were used as reference. As a result, we could identify the miRNA from these samples.
2018-12-10 | GSE121156 | GEO
Project description:Stem cell exosome small RNA sequencing
Project description:This project provides untargeted metabolomics profiling data of fresh winter melon (Benincasa hispida) juice exosome-like nanovesicles. The dataset includes raw mass spectrometry files, metabolite identification results and quantitative data. It complements our previously deposited small RNA sequencing data (NCBI BioProject: PRJNA1475232) and lipidomics data of the same batch of samples, forming a complete multi-omics resource for plant exosome research.