Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identifying the transcriptional targets of the Drosophila transcription factor Ets21c via Targeted DamID

ABSTRACT: Identifying the transcriptional targets of the Drosophila transcription factor Ets21c via Targeted DamID

PROVIDER: PRJNA503866 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8159000.fastq.gz	Fastqsanger.gz
	SRR8159010.fastq.gz	Fastqsanger.gz
	SRR8159001.fastq.gz	Fastqsanger.gz
	SRR8159011.fastq.gz	Fastqsanger.gz
	SRR8159002.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Identifying the transcriptional targets of the Drosophila transcription factor Ets21c via Targeted DamID

Project description:Tissue homeostasis relies on stem cell activity which drives replacement of differentiated cells to maintain tissue integrity and function. The stress-inducible transcription factor Ets21c promotes tissue renewal in the Drosophila adult intestine by controlling intestinal stem cell proliferation and apoptosis of mature enterocytes. To determine how Ets21c fulfills these distinct tasks in the different cell types of the gut, we exploited a genome-wide in vivo Targeted DamID mapping approach to identify its putative direct targets. In young unstressed midguts, Ets21c contacts regulatory loci occupied by as well as devoid of the Polymerase II binding. Follow-up functional genetic studies in intestinal stem cells and enterocytes demonstrate that Ets21c controls cell type-specific sets of target genes that orchestrate cellular behavior via intrinsic and non-autonomous signaling mechanisms. Ets21c thus emerges as a vital determinant of adult health and lifespan.

2019-06-04 | GSE122170 | GEO

Identifying targets of DSX with DamID-seq

Project description:We have adapted the DamID protocol for use with high throughput sequencing. We have used DamID to identify the positions within the Drosophila genome where the transcription factor DSX is bound. We sequenced DpnI-digested genomic DNA from fly tissues containing UAS-Dam (control) or UAS-Dam-DsxF or UAS-Dam-DsxM.

2014-10-30 | GSE27269 | GEO

Identifying targets of DSX with DamID-seq

2014-10-30 | E-GEOD-27269 | biostudies-arrayexpress

Identifying targets of DSX with DamID-chip

Project description:We have used microarrays to identify DSX occupancy signal in adult female fatbody using the DamID protocol. We have performed DamID-chip on adult female fatbody with three biological replicates.

2014-10-30 | E-GEOD-49478 | biostudies-arrayexpress

Identifying targets of DSX with DamID-chip

Project description:We have used microarrays to identify DSX occupancy signal in adult female fatbody using the DamID protocol.

2014-10-30 | GSE49478 | GEO

damidseq_pipeline: an automated pipeline for processing DamID sequencing datasets

Project description:DamID is a powerful technique for identifying regions of the genome bound by a DNA-binding (or DNA-associated) protein. Currently no method exists for automatically processing next-generation sequencing DamID (DamID-seq) data, and the use of DamID-seq datasets with normalisation based on read-counts alone can lead to high background and the loss of bound signal. DamID-seq thus presents novel challenges in terms of normalisation and background minimisation. We describe here damidseq_pipeline, a software pipeline that performs automatic normalisation and background reduction on multiple DamID-seq FASTQ or BAM datasets. Single replicate profiling of pol II occupancy in 3rd instar larval neuroblasts of Drosophila

2015-06-26 | E-GEOD-69184 | biostudies-arrayexpress

Identifying targets of the zinc finger transcription factor Salm during Drosophila myogenesis

Project description:Identifying targets of the zinc finger transcription factor Salm during Drosophila myogenesis

| PRJNA815349 | ENA

Genome-winding binding profile of KDM5 in Drosophila larval prothoracic gland cells via Targeted DamID analysis

Project description:kdm5 is an essential gene in Drosophila that has critical developmental roles in the prothoracic gland cells of the larval ring gland. To profile KDM5 binding within these cells and this developmental stage, we performed Targeted DamID (TaDa). By profiling the nearest genes to significant TaDa peaks (FDR < 0.01), we identified 5815 candidate KDM5 target genes. Interestingly, 42% of these candidate KDM5 target genes appear to be conserved across multiple cellular contexts in Drosophila and span many cellular processes for future investigation.

2023-10-16 | GSE229076 | GEO

DamID data for Comr binding in drosophila testes

Project description:Comr protein was found to be a major regulator of gene activity in drosophila spermatocytes. We obtained Comr binding profile to determine targets of Comr. Comr binding in drosophila male germ line cells was determined using DamID technique.

2013-08-13 | GSE49799 | GEO

Targeted DamID-seq of the Drosophila carpet glia

Project description:Targeted DamID-seq of the Drosophila carpet glia

| PRJEB30096 | ENA