Project description:To reveal the transcriptomes associated with Maresin 1-treated Kupffer cells, the primary Kupffer cells isolated from mouse liver were treated with maresin 1 and the gene expression patterns were analyzed by microarray.
Project description:To reveal the transcriptomes associated with M1 or M2-polarized Kupffer cells, the primary Kupffer cells isolated from mouse liver were treated with lipopolysaccharides or IL-4 and the gene expression patterns were analyzed by microarray. To study the role of RORα in Kupffer cell polarization, Kupffer cells were treated with RORα ligands and transcriptions were compared with those of the M1/M2 polarized Kupffer cells.
Project description:Ulcerative colitis impact on Kupffer cell homeostasis was assessed using transcriptional analysis. Kupffer cells were sorted from the livers of mice treated with dextran sodium sulphate for 7 days. Total RNA preparation was performed on 20,000 sorted Kupffer cells.
Project description:Maresin 1 administered to mice prior to orthopedic surgery exerts distinct anti-inflammatory and pro-resolving effects through regulation of MΦ infiltration, NF-κB signaling, and cytokine release. We used microarrays to detail the global programme of gene expression underlying Maresin 1 effect on mouse hippocampus and identified distinct classes of dysregulated genes during this process.
Project description:Kupffer cells were sorted from the liver of WT mice and Atg5-specific deficient Kupffer cells mice. RNAs were extracted from the Kupffer cells and we performed an RNA-seq on these cells.