Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-Seq of PreCFU-E and CFU-E progenitors from wild type and Scf mutants

ABSTRACT: RNA-Seq of PreCFU-E and CFU-E progenitors from wild type and Scf mutants

PROVIDER: PRJNA505314 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8181540.fastq.gz	Fastqsanger.gz
	SRR8181541.fastq.gz	Fastqsanger.gz
	SRR8181532.fastq.gz	Fastqsanger.gz
	SRR8181542.fastq.gz	Fastqsanger.gz
	SRR8181533.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

RNA-Seq of PreCFU-E and CFU-E progenitors from wild type and Scf mutants

Project description:It has been shown previously that endothelial cells and LepR+ stromal cells are the main sources of SCF in vivo in the mouse bone marrow. We tested whether SCF from endothelial cells and/or LepR+ stromal cells is important for the maintenance of hematopoietic progenitors and erythroid progenitors in mouse bone marrow by conditional deletion of Scf from these two cell types. We discovered that Scf deletion from LepR+ stromal cells, but not endothelial cells, reduced the numbers of hematopoietic progenitors and erythroid progenitors in mice. We performed RNA-Seq on PreCFU-E and CFU-E progenitors from control mice and from mice with Scf deletion from LepR+ stromal cells. We discovered that lack of SCF from LepR+ cells induces a premature differentiation of PreCFU-E and CFU-E progenitors.

2018-12-09 | GSE122468 | GEO

Interacting proteins of NF2 wild-type and mutants

Project description:NF2 wild-type and mutants were re-expressed in HEK29T NF2 knockout cells; We did IP-Mass spectrum for detecting their interacting proteins.

2021-06-23 | PXD026870 |

Isolation and Transcriptome Analyses of Human Erythroid Progenitors: BFU-E and CFU-E

Project description:Burst-forming unit-erythroid (BFU-E) and colony-forming unit-erythroid (CFU-E) cells are erythroid progenitors traditionally defined by colony assays. We developed a flow cytometry-based strategy for isolating human BFU-E and CFU-E cells based on the changes in expression of cell surface markers during in vitro erythroid cell culture. BFU-E and CFU-E are characterized by CD45+GPA-IL-3R-CD34+CD36-CD71low and CD45+GPA-IL-3R-CD34-CD36+CD71high phenotypes, respectively. Colony assays validated phenotypic assignment giving rise to BFU-E and CFU-E colonies, both at a purity ~90%. The BFU-E colony forming ability of CD45+GPA-IL-3R-CD34+CD36-CD71low cells required SCF and erythropoietin, while the CFU-E colony forming ability of CD45+GPA-IL-3R-CD34-CD36+CD71high cells required only erythropoietin. Bioinformatic analysis of the RNA-seq data revealed unique transcriptomes in each differentiation stage. The sorting strategy was validated in uncultured primary cells isolated from bone marrow and peripheral blood, indicating that marker expression is not an artifact of in vitro cell culture, but represents an in vivo characteristic of erythroid progenitor populations. The ability to isolate highly pure human BFU-E and CFU-E progenitors will enable detailed cellular and molecular characterization of these distinct progenitor populations and define their contribution to disordered erythropoiesis in inherited and acquired hematological disease. Our data provide important resource for future studies. Transcription profiles of Human erythroid progenitors at distinct developmental stages were generated by deep sequencing, in triplicate, using IlluminaHiSeq 2000. The complete dataset comprises 4 sample types: CD34, BFU, CFU, and Pro (reanalysis of GSM1304777-GSM1304779).

2014-10-30 | E-GEOD-61566 | biostudies-arrayexpress

Isolation and Transcriptome Analyses of Human Erythroid Progenitors: BFU-E and CFU-E

2014-10-30 | GSE61566 | GEO

lin-35(n745) mutants vs. wild type

Project description:Transcriptional profiling of C. elegans first larval stage whole animals comparing lin-35(n745) mutants and N2 (wild type) at 26 degrees.

2011-01-25 | GSE26824 | GEO

lin-15B(n744) mutants vs. wild type

Project description:Transcriptional profiling of C. elegans first larval stage whole animals comparing lin-15B(n744) mutants and N2 (wild type) at 26 degrees.

2011-01-25 | GSE26823 | GEO

Arabidopsis thaliana seedlings: Wild type vs. zrf1 mutants

Project description:Transcriptional profiling of 6-day-old seedlings of Arabidopsis wild type control and zrf1 mutants is performed using Agilent's Whole Arabidopsis Gene Expression Microarray (4x44K).

2016-12-31 | GSE76244 | GEO

Transcriptomic analysis of wild type and Nr4a1-/- adipose progenitors.

Project description:Transcriptomic analysis of wild type and Nr4a1-/- adipose progenitors.

| PRJNA487092 | ENA

Transcriptomic comparison of Botrytis cinerea wild type and VELVET mutants

Project description:An RNAseq-based transcriptomic comparison of Botrytis cinerea wild type and the three VELVET mutants was performed, to correlate common virulence defects of the mutants with changes in gene expression. A large number of genes encoding infection-related and secreted proteins was strongly underexpressed in the mutants, in particular genes encoding proteases and carbohydrate active enzymes. Many VELVET-regulated genes were found to be upregulated in the wild type during host plant infection (Müller et al.: Investigations on VELVET regulatory mutants confirm the role of host tissue acidification and secretion of proteins in pathogenesis of Botrytis cinerea; New Phytologist, 2018)

2018-04-02 | GSE112580 | GEO

Transcriptomic Analysis of Wild Type and FOXA2-/- ES-derived Pancreatic Progenitors

Project description:Transcriptomic Analysis of Wild Type and FOXA2-/- ES-derived Pancreatic Progenitors

2019-05-14 | GSE114099 | GEO