ABSTRACT: Isolation of the first New Delhi metallo-ß-lactamase-1 (NDM-1) producing, colistin resistant, Klebsiella pneumoniae sequence type ST15, from a digestive carrier in Albania, May 2018
Project description:The periplasm of gram-negative bacteria facilitates critical functions, including nutrient uptake, cell wall metabolism, antibiotic resistance, and virulence. Efficient quality control of proteins involved in these processes is crucial for bacterial fitness and survival. The limited size of the periplasm has hindered high-resolution mechanistic investigations of complex processes within this compartment. Using in-cell NMR spectroscopy, we dissected the mechanism of periplasmic quality control of the metallo-β-lactamase NDM-1 under conditions of zinc starvation, which destabilizes its native structure promoting its degradation. We show that the protease Prc targets membrane-bound NDM-1 at specific residues and secondary structure motifs, while DegP processes peptides generated by Prc. This approach discloses the concerted mechanism of these proteases at atomic resolution in the periplasm of live cells.
2025-07-10 | PXD065625 | Pride
Project description:Deep mutational scanning of NDM-1 metallo-beta-lactamase
| PRJNA974578 | ENA
Project description:New Delhi metallo-beta-lactamase Klebsiella pneumoniae strains from clinical isolates
Project description:Identification of plasmid-mediated tigecycline resistance tet(x4) and New Delhi Metallo-beta-lactamase (NDM) in an Escherichia coli isolate from Canada