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WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis

ABSTRACT: WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis

PROVIDER: PRJNA505703 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8192660.fastq.gz	Fastqsanger.gz
	SRR8195640.fastq.gz	Fastqsanger.gz
	SRR8195650.fastq.gz	Fastqsanger.gz
	SRR8192661.fastq.gz	Fastqsanger.gz
	SRR8195641.fastq.gz	Fastqsanger.gz

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Similar Datasets

WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis [RNA-seq]

Project description:During development and growth, dynamic signals need to be translated into spatially precise and temporally stable gene expression states, which define cell fate. In the context of the apical plant stem cell system, local accumulation of the small, highly mobile phytohormone auxin triggers organ initiation. Here we show that the WUSCHEL transcription factor locally protects stem cells from differentiation by controlling the auxin signaling and response pathways via regulation of Histone acetylation. Conversely, low levels of signaling are required for stem cell maintenance, demonstrating that WUSCHEL acts as a rheostat on the auxin pathway. Our results reveal an important mechanism that allows cells to differentially translate a potent and highly mobile developmental signal into appropriate cell behavior with high spatial precision and temporal robustness.

2019-11-11 | GSE122610 | GEO

WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis [ChIP-seq]

2019-11-11 | GSE122579 | GEO

WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis [RNA-seq]

Project description:WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis [RNA-seq]

| PRJNA505705 | ENA

WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis [ChIP-seq]

Project description:WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis [ChIP-seq]

| PRJNA505666 | ENA

WUSCHEL acts as an auxin response rheostat to maintain apical stem cells in Arabidopsis.

Project description:To maintain the balance between long-term stem cell self-renewal and differentiation, dynamic signals need to be translated into spatially precise and temporally stable gene expression states. In the apical plant stem cell system, local accumulation of the small, highly mobile phytohormone auxin triggers differentiation while at the same time, pluripotent stem cells are maintained throughout the entire life-cycle. We find that stem cells are resistant to auxin mediated differentiation, but require low levels of signaling for their maintenance. We demonstrate that the WUSCHEL transcription factor confers this behavior by rheostatically controlling the auxin signaling and response pathway. Finally, we show that WUSCHEL acts via regulation of histone acetylation at target loci, including those with functions in the auxin pathway. Our results reveal an important mechanism that allows cells to differentially translate a potent and highly dynamic developmental signal into stable cell behavior with high spatial precision and temporal robustness.

| S-EPMC6841675 | biostudies-literature

WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-11-11 | GSE122611 | GEO

Response of cauline buds to apical auxin and basal cytokinin treatment

Project description:Identification of genes regulated by apical auxin and basal cytokinin treatment of the nodal stem in cauline buds of Arabidopsis thaliana

2016-12-25 | GSE59741 | GEO

E-GEOD-122610

Project description:WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis [RNA-seq]

2020-04-14 | E-GEOD-122610 | ExpressionAtlas

Identification of WUSCHEL direct targets.

Project description:Cell-cell communication is critical for stem cell maintenance. Shoot apical meristem (SAM) located at the shoot tip harbors stem cells within the central zone (CZ). Their progeny differentiate in the adjacent peripheral zone (PZ). WUSCHEL (WUS) is a homeodomain transcription factor produced in a few cells of the organizing center (OC), located beneath the CZ. It has been shown to specify stem cell fate and also activate CLAVATA3 (CLV3) expression in cells of the CZ. CLV3 is a secreted peptide that activates a membrane bound receptor kinase-CLAVATA1 to restrict WUS transcription to the OC. It has been hypothesized that WUS activates CLV3 expression and stem cell fate in adjacent cells of the CZ by activating a non-cell autonomous signal. Contrary to this hypothesis, here we show that the WUS protein after being synthesized in cells of the OC, migrates into the superficial cell layers of the CZ where it activates CLV3 transcription by binding to its promoter elements. WUS also migrates laterally into the PZ to repress the expression of differentiation promoting transcription factors by binding to their regulatory regions. Migration of a stem cell inducing transcription factor into adjacent cells to activate a negative regulator, whereby restricting its own accumulation is unique to plant stem cell niches. While stem cell promoting transcription factor directly repressing differentiation promoting transcription factors to prevent premature differentiation of stem cell progenitors is conserved among diverse stem cell niches. A dexamethasone inducible version of WUSCHEL was used to identify its direct tragets.

2013-03-26 | GSE29364 | GEO

Analysis of the effect of auxin biosynthesis inhibitor L-AOPP on the gene expression profile (shoot apical meristem)

Project description:Recently, 2-aminoxy-3-phenylpropionic acid (L-AOPP) had been demonstrated to possess an inhibitory activity against IAA biosynthesis but the molecular basis of the action was unclear. To investigate the function of L-AOPP, we conducted microarray analysis using the shoot apical meristem (SAM) part of A. thaliana in addition to whole plants after the treatment of L-AOPP. We performed microarray analysis using the shoot apical meristem (SAM) part of A. thaliana (Columbia-0) to investigate function of 2-aminoxy-3-phenylpropionic acid (L-AOPP) in relation to inhibition of auxin biosynthesis. Total RNA was extracted from SAM part of 7-day-old seedlings grown on 1/2 MS medium containing L-AOPP (50 µM),L-AOPP (50 µM) + Indole-3-acetic acid (IAA 10 nM) or Dimethyl sulfoxide (DMSO 0.1%).

2015-12-31 | E-GEOD-30092 | biostudies-arrayexpress

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