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Mus musculus

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Atg7-/- bone marrow LSK(Lin-scal-1+c-kit+) cells Transcriptomes

PROVIDER: PRJNA506815 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR8241920_1.fastq.gz	Fastqsanger.gz
	SRR8241920_2.fastq.gz	Fastqsanger.gz
	SRR8241915_1.fastq.gz	Fastqsanger.gz
	SRR8241915_2.fastq.gz	Fastqsanger.gz
	SRR8241916_1.fastq.gz	Fastqsanger.gz

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Project description:The study was a comparison of gene expression using RNA-seq. We analyzed the stem and progenitor cells from WT and Vav-cre+ Tet2fl/fl Flt3-ITD (T2F3) mice. We isolated stem cells LSK (lin- sca+ kit+) and granulocyte-macrophage progenitors GMP (lin- sca- kit+ fcgr+ cd34+) cells from bone marrow. Comparisons were made across genotypes WT vs. T2F3 and cell types LSK vs. GMP.

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Project description:compare the gene expression profile between irradiated Lin-Sca-1+c-Kit+ (LSK) cells from mouse bone marrow reconstituted with wild type and necdin null fetal liver cells The Affymetrix oligonucleotide array was used for this analysis compare the gene expression profile betweenirradiated Lin-Sca-1+c-Kit+ (LSK) cells from mouse bone marrow reconstituted with wild type and necdin null fetal liver cells

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Project description:The gene expression of bone marrow Hdc-/- and WT (LSK, Lin-c-kit+Sca-1+) hematopoetic stem and progenitor cells were isolated from Hdc-/- or WT mice. Cells were sorted by the cell surface markers of LSK total RNA was isolated from sorted 2,000 HSPCs using the ARCTURUS PicoPure RNA isolation kit (Life Technologies). cDNA was amplified and libraries were constructed by using the SMARTer Ultra Low Input RNA kit (Clontech Laboratories) and the Nextera XT DNA Library Preparation kit (Illumina) according to the respective manufacturer’s instructions. Sequencing was performed on the Illumina HiSeq2500 platform.

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