Behavioral and neurogenomic responses to acoustic and visual sexual cues are correlated in female torrent frogs
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA506982 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR8244410_1.fastq.gz | Fastqsanger.gz | |||
| SRR8244410_2.fastq.gz | Fastqsanger.gz | |||
| SRR8244411_1.fastq.gz | Fastqsanger.gz | |||
| SRR8244411_2.fastq.gz | Fastqsanger.gz | |||
| SRR8244412_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 30 |
Similar Datasets
Project description:Diverse animal species use multimodal communication signals to coordinate reproductive behavior. Despite active research in this field, however, the brain mechanisms underlying multimodal communication remain poorly understood. Although anuran amphibians rely on acoustic communication some anurans like humans and many mammalian species, often produce auditory signals accompanied by conspicuous visual signals (e.g. vocal-sac inflation). In this study, we used video playbacks to determine the role of vocal-sac inflation in little torrent frogs (Amolops torrentis). The results showed that auditory cues (i.e. male advertisement calls) as well as visual cues are attractive to female torrent frogs, although the auditory cue was more attractive than the visual cue. Moreover, females preferred simultaneous bimodal cues to unimodal cues. Then we exposed females to blank, visual, auditory and audiovisual stimuli and analyzed whole brain tissue gene expression changes using RNA-seq. The hierarchical clustering of differentially expressed genes (DEGs) showed that samples from the same behavioral stimulus category were more aggregative than those from different behavioral categories nearly forming a cluster, and implying a strong relationship between neurogenomic states and momentarily expressed sexual signals. We also found that the Gene Ontology (GO) terms and KEGG pathways involved in energy metabolism were mostly enriched in blank contrast versus visual, acoustic or audiovisual stimuli, suggesting that brain energy use plays a key role in the perception of these stimuli. Moreover, analyses of immediate early genes (IEGs) showed that only the c-fos gene was differently expressed in response to different stimuli. These results increase our understanding of how neurogenomic states reflect brain mechanisms mediating female preferences for different sexual cues.
2020-11-24 | GSE122947 | GEO
Project description:Many animals rely on complex signals that target multiple senses to attract mates and repel rivals. These multimodal displays can however also attract unintended receivers, which can be an important driver of signal complexity. Despite being taxonomically widespread, we often lack insight into how multimodal signals evolve from unimodal signals and in particular what roles unintended eavesdroppers play. Here, we assess whether the physical movements of parasite defense behavior increase the complexity and attractiveness of an acoustic sexual signal in the little torrent frog (Amolops torrentis). Calling males of this species often display limb movements in order to defend against blood-sucking parasites such as frog-biting midges that eavesdrop on their acoustic signal. Through mate choice tests we show that some of these midge-evoked movements influence female preference for acoustic signals. Our data suggest that midge-induced movements may be incorporated into a sexual display, targeting both hearing and vision in the intended receiver. Females may play an important role in incorporating these multiple components because they prefer signals which combine multiple modalities. Our results thus help to understand the relationship between natural and sexual selection pressure operating on signalers and how in turn this may influence multimodal signal evolution.
| S-EPMC9122496 | biostudies-literature
Project description:Zhao et al. recently reported results which, they claim, suggest that sexual selection produces the multimodal displays seen in little torrent frogs (Amolops torrentis) by co-opting limb movements that originally evolved to support parasite defense (Zhao et al., 2022). Here, we explain why we believe this conclusion to be premature.
| S-EPMC10561974 | biostudies-literature
Project description:Recently we showed that limb movements associated with anti-parasite defenses can enhance acoustic signal attraction in male little torrent frogs (Amolops torrentis), which suggests a potential pathway for physical movements to become co-opted into mating displays (Zhao et al., 2022). Anderson et al. argue for alternative explanations of our results and provide a reanalysis of part of our data (Anderson et al., 2023). We acknowledge some of the points raised and provide an additional analysis in support of our hypothesis.
| S-EPMC10561973 | biostudies-literature
Project description:Eukaryotic cells are equipped with multiple mechanosensing systems and perceive wide range of mechanical stimuli from the environment. However, cell-level responses against acoustic waves, which transmits feeble but highly frequent physical perturbation, largely remains uninvestigated, especially with regard to audible range of sound. Here we investigate the effect of acoustic stimulation on gene expression profiles of mammalian cultured cells. A direct sound emission system was set up using a vibrational transducer to directly generate acoustic waves in culture medium. A custom-made vibrating plate made of PEEK (poly ether ether ketone) plastic was used as a diaphragm. A set of sound patterns including single-frequency sound and white noise were generated by NCH Tone Generator software. Sound intensity was directly measured by recording it in water using a hydrophone and the pressure level was calculated. C2C12 myoblasts cultured in a plastic dish with approximately 50% confluency were subjected to acoustic stimulation. 440 Hz and 14k Hz single-frequency sine wave sound were selected as representatives of low and high audible frequencies, and white noise was selected as a random noise pattern. 2 and 24 hours after continuous emission of these sound at 100 Pa, total RNA was extracted and subjected to the gene expression profiling analysis by RNA-sequencing technique. Total 42 early- and 145 late-response genes were identified as sound-sensitive genes in 2 and 24 hours stimulation, respectively. Gene annotation analyses revealed that in addition to the known mechanosensitive activities such as fluid shear stress response, cell migration, cell adhesion and blood vessel development, variety of pathways and processes were identified to be affected by acoustic stimulation.
2025-03-12 | GSE247726 | GEO
Project description:Bacteria are known to respond to various environmental stimuli including nutrient deprivation, osmotic stress, and exposure to antibiotics. Our experimental data showed that P. aeruginosa biofilm formed in urinary catheters is very suseptible to gentamicin treatment when combined with exposure to surface acoustic waves Our goal was to establish whether P. aeruginosa is capable of specifically sensing the surface acoustic waves as well as to try and decipher the molecular mechanism underlying the increased biofilm suseptibility to antibiotics treatment
2011-09-01 | GSE28081 | GEO
Project description:Motherhood is characterized by dramatic changes in brain and behavior, but less is known about fatherhood. Here we report that male sticklebacks – a small fish in which fathers provide care – experience dramatic changes in neurogenomic state as they become fathers. Some genes are unique to different stages of paternal care, some genes are shared across stages, and some genes are added to the previously acquired neurogenomic state. Comparative genomic analysis suggests that some of these neurogenomic dynamics resemble changes associated with pregnancy and reproduction in mammalian mothers. Moreover, gene regulatory analysis identified transcription factors that are regulated in opposite directions in response to a territorial challenge versus during paternal care. Altogether these results show that some of the molecular mechanisms of parental care might be deeply conserved and might not be sex-specific, and suggest that tradeoffs between opposing social behaviors are managed at the gene regulatory level.
2019-09-15 | GSE134508 | GEO
Project description:Nanoscale Acoustic Oscillator for Mechanoimmunology: NAOMI
| PRJNA1294740 | ENA
Project description:Foragers rely on various cues to assess predation risk. Information theory predicts that high certainty cues should be valued more than low certainty cues. We measured the latency of black-capped chickadees (Poecile atricapillus) to resume feeding during winter in response to cues that conferred different degrees of certainty about current predation risk: a high certainty visual cue (predator mount) and a lower certainty acoustic cue (conspecific mobbing calls), presented either alone or in combination. As predicted, chickadees took longer to resume feeding after the visual than the acoustic cue, and this effect was greatest under conditions of high starvation risk (i.e. low temperatures). Presenting both cues together produced the same foraging delay as the visual cue alone under low starvation risk, but surprisingly, resulted in lower responses under high starvation risk compared to the visual cue alone. We suggest that this may be due to prey using a form of information updating, whereby differences in the timing of perception of acoustic versus visual cues interacts with energetic constraint to shape perceived risk. Although the sequential perception of cues is likely in a range of decision-making contexts, studies manipulating the order in which cues are perceived are needed to test existing models of multimodal cue integration.
| S-EPMC7657859 | biostudies-literature
Project description:Mechanoimmunology provides a powerful framework for understanding how mechanical forces orchestrate immune responses, offering insights into immune cell functions and the mechanisms underlying mechanotransduction. However, a major challenge in this field is the absence of reliable platforms that deliver precise and consistent mechanical stimuli to individual cells while achieving practical and reproducible mechanobiological activation of immune cells. In this work, we introduce a highly sensitive and stable nanoscale acoustic oscillator for mechanoimmunology applications: NAOMI. NAOMI features a micropatterned substrate that promotes uniform cell monolayer formation, seamlessly integrated with an acoustic transducer system capable of generating precise nanoscale oscillations (±1 nm deviation) for up to 72 h of continuous stimulation. Unlike conventional passive mechanobiological platforms that rely on static stiffness or topographical cues, NAOMI enables programmable, uniform 3D oscillations, delivering finely tuned and reproducible mechanical stimulation for diverse experimental applications. Validation studies demonstrate that NAOMI-induced oscillations significantly amplify stress intensity and cell displacement, driving robust M1 pro-inflammatory polarization in macrophages. With its high precision, stability, and tunability, NAOMI offers a powerful and versatile platform for studying mechanoimmunology and holds strong potential to support future research and enable innovative immunotherapy applications.
2025-08-20 | GSE303278 | GEO