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ABSTRACT: 16S Metabarcoding analysis of native vs. sand-amended salt marsh sediments

PROVIDER: PRJNA507114 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8246780.fastq.gz	Fastqsanger.gz
	SRR8246790.fastq.gz	Fastqsanger.gz
	SRR8246781.fastq.gz	Fastqsanger.gz
	SRR8246791.fastq.gz	Fastqsanger.gz
	SRR8246782.fastq.gz	Fastqsanger.gz

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Project description:Salt marshes provide many key ecosystem services that have tremendous ecological and economic value. One critical service is the removal of fixed nitrogen from coastal waters, which limits the negative effects of eutrophication resulting from increased nutrient supply. Nutrient enrichment of salt marsh sediments results in higher rates of nitrogen cycling and, commonly, a concurrent increase in the flux of nitrous oxide, an important greenhouse gas. Little is known, however, regarding controls on the microbial communities that contribute to nitrous oxide fluxes in marsh sediments. To address this disconnect, we generated microbial community profiles as well as directly assayed nitrogen cycling genes that encode the enzymes responsible for overall nitrous oxide flux from salt marsh sediments. We hypothesized that communities of microbes responsible for nitrogen transformations will be structured by nitrogen availability. Taxa that respond positively to high nitrogen inputs may be responsible for the elevated rates of nitrogen cycling processes measured in fertilized sediments. Our data show that, with the exception of ammonia-oxidizing archaea, the community composition of organisms responsible for production and consumption of nitrous oxide was altered under nutrient enrichment. These results suggest that elevated rates of nitrous oxide production and consumption are the result of changes in community structure, not simply changes in microbial activity.

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