Project description:We analyzed lncRNAs located in CNV loci associated with congenital anomalies of the kidney and urinary tract (CAKUT). HSALNG0134318 at CNV 22q11 was identified as CAKUT related lncRNA, which was coexpressed with multiple known CAKUT associated genes. To validate our findings, we performed knockdown experiments in HEK293 cell line to characterize the trascriptomic profiles regulated by HSALNG0134318 in human embryonic kidney cells.
Project description:Bioinformatics analysis combined with in vitro and in vivo experiments revealed the characteristics of intestinal flora and metabolic differences in patients with gestational diabetes mellitus (GDM), and the mechanism of affecting gpr43 pathway and thus affecting the offspring's congenital anomalies of the kidney and the urinary tract (CAKUT)