Project description:The Long Evans/orl (LE/orl) rat is an animal model of inherited undescended testis (UDT). To explore genetic mechanisms of UDT, we studied differential gene expression in LE/orl and LE wild type (LE/wt) fetal gubernaculum and testis. Analyses of differentially expressed probe sets linked below as supplementary files: Analysis_methods.txt [A description of the analysis] gub.txt [Genes differentially expressed in between wt and orl gubernacula] testes.txt [Genes differentially expressed between wt and orl testes] wt.txt [Genes differentially expressed in wt gubernacular development] Keywords: strain/condition predisposition, disease state analysis, developmental
Project description:The main goal of this study was to characterize the Dredd-independent functions of PGRP-LE in the adult Drosophila midgut (the midgut is the functional analog of the vertebrate small intestine). We expressed a UAS-PGRP-LE transgene under the control of mex-GAL4, which is specifically active in midgut enterocytes, in a Dredd mutant background. We then collected adult male midguts, extracted total RNA and performed mRNA-seq. Our data show that PGRP-LE overexpression alters fundamental aspects of midgut metabolism in Dredd mutant midguts.