Ontology highlight
ABSTRACT:
PROVIDER: PRJNA507961 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR8271630.fastq.gz | Fastqsanger.gz | |||
| SRR8271640.fastq.gz | Fastqsanger.gz | |||
| SRR8271650.fastq.gz | Fastqsanger.gz | |||
| SRR8271631.fastq.gz | Fastqsanger.gz | |||
| SRR8271641.fastq.gz | Fastqsanger.gz |
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Project description:The oomycete P. oligandrum is a soil-inhabiting parasite and predator of both fungi and oomycetes and uses hydrolytic enzymes extensively to penetrate and hydrolyze its host or prey. Other mechanisms have been overlooked, and we investigated whether P. oligandrum-produced volatile organic compounds (VOCs) could also be contributing to antagonism. VOCs have diverse functions, including contributing to antagonism in ecological interactions and potential applications in biocontrol. The growth-inhibiting activity of P. oligandrum VOCs was tested on P. myriotylum – a host or prey of P. oligandrum – coupled with electron microscopy, biochemical assays and transcriptomic analysis. The total VOCs produced by P. oligandrum reduced P. myriotylum growth by 80% and zoospore levels by 60%. GC-MS identified twenty-three VOCs, and methyl heptenone, D-limonene, 2-undecanone and 1-octanal were potent inhibitors of P. myriotylum growth and led to increased production of reactive oxygen species at a concentration that did not inhibit P. oligandrum growth. Exposure to the total VOCs of P. oligandrum led to shrinkage of P. myriotylum hyphae, and lysis of the cellular membranes and organelles. Transcriptomic analysis of P. myriotylum exposed to the P. oligandrum VOCs at increasing levels of growth inhibition showed initially a strong upregulation of putative detoxification related genes that was not maintained later during exposure to the VOCs. The inhibition of P. myriotylum growth continued after the exposure to the VOCs was discontinued and led to reduced leaf lesion size during P. myriotylum infection of its plant host. The VOCs produced by P. oligandrum could be another factor alongside hydrolytic enzymes contributing to its ecological role as a microbial antagonist where the concentration of the VOCs may be inhibitory in particular ecological niches such as in soil. The VOCs analyzed here may also be contributing to the biocontrol of diseases using P. oligandrum commercial preparations.
2022-12-31 | GSE214639 | GEO
Project description:Previously, we investigated the effect of fungal VOCs on the behavior of phylogenetically different soil bacteria (Schmidt et al 2015). In these experiments we showed that VOCs emitted by several fungi can lead to phenotypical responses in bacteria, for example, by inducing a change in motility (Schmidt et al 2015). We observed that the plant pathogenic fungus Fusarium culmorum produced a unique cluster of VOCs consisting primarily of terpenes. When exposed to the VOCs emitted by this fungus, the rhizobacterium Serratia plymuthica PRI-2C responded with an induction of motility. It is plausible that in soil, microorganisms sense changes in their environments via shifts in VOCs blend and adapt their behavior accordingly (Garbeva et al 2014). Although several studies indicated that VOCs can be used as signaling molecules in microbial inter-species interactions, the following questions remain unanswered as how are VOCs perceived as signals by the microorganisms and which regulatory pathways and genes are involved in the response? To answer these questions, the rhizosphere isolate S. plymuthica PRI-2C was grown alone or exposed to VOCs emitted by F. culmorum. The bacterial transcriptome and proteome were analyzed under each situation to identify the molecular basis of the bacterial response to fungal VOCs.
2018-07-19 | PXD004819 | Pride
Project description:Sugarcane holds substantial agricultural and industrial importance in Brazil and worldwide, primarily due to its use in biofuel production and for a range of biomolecules of diverse biochemical applications. The drive towards sustainable agricultural practices has fostered the interest in the exploration of bioproducts to reduce the dependence on agrochemicals. In this context, microbial volatile organic compounds (VOCs) have emerged as potential biofungicides, offering several advantages over conventional chemical treatments. This study focused on evaluating their antagonistic potential against Sporisorium scitamineum, the causative agent of sugarcane smut disease, and investigated the molecular mechanisms underlying their inhibitory effects. Two Pseudomonas bacterial strains isolated from sugarcane roots exhibited remarkable antagonistic activity against S. scitamineum. Notably, ITA P2F2 completely inhibited the growth of the phytopathogen and was identified as a novel species within the Pseudomonas genus. A total of 63 VOCs were identified from both bacterial isolates, with eight showing inhibitory activity against the phytopathogen. Transcriptomic analyses revealed significant changes in gene expression of S. scitamineum when exposed to VOCs from ITA P2F2, including the downregulation of genes involved in central metabolic pathways, such as carbohydrate metabolism and fatty acid β-oxidation, and the upregulation of genes associated with DNA repair. Potential DNA damage caused by the VOCs was further evidenced through temporal analysis using Fourier Transform Infrared (FTIR) spectroscopy. Additionally, electron microscopy analysis showed structural damage to the fungal hyphae after VOCs exposure. This multidisciplinary study is the first to investigate the inhibition of S. scitamineum using VOCs. Our findings contribute to the molecular understanding of these signaling molecules and highlight their potential as promising bioproducts to replace conventional agrochemicals in the control of sugarcane pathogens.
2025-11-10 | GSE279266 | GEO
Project description:VOCs influence microbial diversity and community composition in soils from Tibet.
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Project description:We identified and validated characteristic miRNA expression profiles of human whole blood in workers exposed to volatile organic compounds (VOCs) and compared the usefulness of miRNA indicator of VOCs with the effectiveness of the already used urinary biomarkers of occupational exposure.
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