Lignin degradation active genes studies in aquatic environment
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PROVIDER: PRJNA511352 | ENA |
REPOSITORIES: ENA
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Project description:lignin degradation genes studies in aquatic environment Metagenome
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Project description:lignin degradation genes and groups studies in aquatic environment Raw sequence reads
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Project description:Consolidated bioprocessing (CBP) represents a promising approach to convert biomass into valuable chemicals by integrating the hydrolysis of cellulose and hemicellulose with microbial fermentation in a single step. This study investigates the potential of CBP technique for the efficient conversion of lignocellulosic biomass into malic acid using the thermophilic fungus Myceliophthora thermophila. Transcriptomic analysis and genetic studies indicated that efficient degradation of lignin is crucial for improving the overall efficiency of CBP, as it facilitates access to cellulose and hemicellulose while enabling the utilization of lignin-derived compounds. The assay of lignin degradation products revealed that M. thermophila can channel main lignin-derived components into the central metabolic pathway for malic acid production. The combined enhancement of lignin degradation capabilities and optimization of the cellodextrin utilization pathway in M. thermophila resulted in 52.9% increase in malic acid production, from 36.3 g/L to 55.5 g/L directly from raw corncob. In a bioreactor, malic acid titer reached 151.2 g/L by one-step bioconversion of plant biomass, with the productivity and yield of 1.09 g/L/h and 0.55 g/g raw corncob. Following separation and purification, the purity of malic acid reached 99%. This study underscores the viability of CBP as a sustainable approach for biobased chemical production and emphasizes the importance of optimizing both lignin degradation and product synthesis pathways to maximize the efficiency of biomass conversion processes.
2026-08-19 | GSE281203 | GEO
Project description:Analysis of Fe ligands in lignin degradation media
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Project description:Lignin is a universal waste product of the agricultural industry and is currently seen as a potential feedstock for more sustainable manufacturing. While it is the second most abundant biopolymer in the world, most of it is currently burned as it is a very recalcitrant material. Many recent studies, however, have demonstrated the viability of biocatalysis to improve the value of this feedstock and convert it into more useful chemicals, such as polyhydroxybutyrate, and clean fuels like hydrogen and n-butanol. Rhodopseudomonas palustris is a gram-negative bacterium which demonstrates a plethora of desirable metabolic capabilities, including aromatic catabolism useful for lignin degradation. This study uses a multi-omics approach, including the first usage of CRISPRi in R. palustris, to investigate the lignin consumption mechanisms of R. palustris, the essentiality of redox homeostasis to lignin consumption, elucidate a potential lignin catabolic superpathway, and enable more economically viable sustainable lignin valorization processes.
2025-08-25 | PXD060837 | Pride
Project description:Salmonella spp., and ESBL-Producing E. coli in Nile perch and aquatic environment of Lake Victoria, Tanzania
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Project description:The Trametes versicolor genome is predicted to encode many enzymes that can effectively degrade lignin, making it a has potentially useful application intool for biopulping and biobleaching. Poplar is an important and widely cultivated species of tree species, which isand extensively applied used in the pulping industry. However, the wood degradation mechanism of T. versicolor from transcriptomic level is not clear. To reveal identify the enzymes that contributeing to lignocellulose degraredauction and its degradation mechanisms, we evaluated transcriptomic how study theof T. versicolor transcriptome was changes during evaluated growthing on the poplar wood relative to growth on glucose medium. 853 genes were differentially expressed;, 360 genes were up-regulated on poplar wood, and 493 genes were down-regulated on poplar wood. Notably, most genes relative involved into lignin degradation were up-regulated, including eight lignin peroxidase (LiP) genes, and two manganese peroxidase (MnP) genes etc. Genes encoding cellulose and hemicelluloses degrading-enzymesation were mostly down-regulated, including six endo-β-1, 4-glucanase genes, three cellobiohydrolase I genes, and one cellobiohydrolase II gene, etc. MeanwhileAdditionally, expression of more significant expansion of P450s in T. versicolor genome, along with differences in carbohydrate- and lignin-degrading enzymes, could bewere correlated withto poplar wood degradation. Our results revealed transcriptomic characterizeation transcriptomic changes related toof lignocellulose degradation. Therefore, our results cwould be benuseful for the development ofefit T. versicolor as a tool to improve the efficiency of lignin degradation, and provide a theoretical foundation for a new paper pulp manufacturing processe 1,T.versicolor groewn on PDA medium. 2, T. versicolor growing on the a glucose carbon medium of glucose. 3, T. versicolor growing on poplar medium
2016-08-06 | E-GEOD-81742 | biostudies-arrayexpress