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LncRNA and mRNA expression in paired liver tissues (periparasitic liver tissues versus distal liver tissues) from alveolar echinococcosis (AE) patients

ABSTRACT: LncRNA and mRNA expression in paired liver tissues (periparasitic liver tissues versus distal liver tissues) from alveolar echinococcosis (AE) patients

PROVIDER: PRJNA511859 | ENA |

REPOSITORIES: ENA

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LncRNA and mRNA expression in paired liver tissues (periparasitic liver tissues versus distal liver tissues) from alveolar echinococcosis (AE) patients

Project description:To determine the LncRNA and mRNA expression profile in periparasitic liver tissues and matched distal-liver tissues, we used LncRNA microarray analysis from Arraystar to examine the expression of LncRNAs and mRNAs in periparasitic liver tissues and matched distal-liver tissues.

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Project description:Alveolar (AE) and cystic (CE) echinococcosis are two parasitic diseases caused by the tapeworms Echinococcus multilocularis and E. granulosus sensu lato (s. l.), respectively. Currently, AE and CE are mainly diagnosed by means of imaging techniques, supported by serology and clinical and epidemiological data. However, no viability markers that indicate parasite state during infection are available. Extracellular small RNAs (sRNAs) are short non-coding RNAs that can be secreted by cells through association with extracellular vesicles, proteins, or lipoproteins. Circulating sRNAs can show altered expression in pathological states; hence, they are intensively studied as biomarkers of several diseases. Here, we profiled the sRNA transcriptomes in the context of AE and CE to identify novel biomarkers to aid in medical decisions. For this, endogenous and parasitic sRNAs were analysed by sRNA sequencing in sera from disease negative, positive, and treated patients and patients harbouring a non-parasitic lesion. Consequently, 20 differentially expressed sRNAs associated with AE, CE and/or non-parasitic liver lesion were identified. Our results represent an in-depth characterisation of the effect E. multilocularis and E. granulosus s.l. exert on the extracellular sRNA landscape in human infections and provide a set of novel candidate biomarkers for both AE and CE detection.

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Project description:Alveolar echinococcosis (AE) is a chronic and potentially fatal zoonotic parasitic disease that seriously affects the host's health. It is caused by the proliferation of Echinococcus multilocularis larvae within the liver. Due to its long incubation period following host infection, early diagnosis of the disease is currently not feasible. Treatment options are extremely limited, with the only choice being curative surgical resection combined with benzimidazole medication. Thus, the development of early, rapid, and minimally invasive diagnostic methods is crucial for enhancing patient prognosis. This study conducted a proteomic and metabolomic investigation into the alterations of proteins and metabolites in AE mouse serum, aiming to explore potential serum biomarkers. Twenty-two proteins exhibited significant differences between the treatment and control groups, primarily involved in carbohydrate metabolism, lipid metabolism, and amino acid metabolism. Upregulation of genes related to immune response and enhanced glycolysis were observed, possibly associated with the reproduction of E. multilocularis in the liver. A total of 182 metabolites were screened to distinguish between AE mice and healthy mice. A significant increase in cP450 metabolite of arachidonic acid indicated signs of renal and splenic involvement in AE mice. Furthermore, KEGG analysis suggested a close association between amino acid metabolism and the occurrence of AE. These data provide a promising avenue for the discovery of biomarkers and the study of potential etiological processes.

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