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Single Cell RNA Sequencing Facilitates Quantitative Analysis of CD31+ endothelial cells at day4 of hESC hematopoietic differentiation

ABSTRACT: Single Cell RNA Sequencing Facilitates Quantitative Analysis of CD31+ endothelial cells at day4 of hESC hematopoietic differentiation

PROVIDER: PRJNA511860 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8369969_1.fastq.gz	Fastqsanger.gz
	SRR8369969_2.fastq.gz	Fastqsanger.gz

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Single Cell RNA Sequencing Facilitates Quantitative Analysis of CD31+ endothelial cells at day4 of hESC hematopoietic differentiation

Project description:Purpose: The goals of this study are to investigate the cell fate desicion and molecular mechanism in CD31+ endothelial cells at the day4 of hematopoietic differentiation. Methods: mRNA profiles of Single CD31+ endothelial cell at day4 of hESC hematopoietic differentiation, were generated by deep sequencing using Illumina GAIIx. The sequence reads that passed quality filters were analyzed with CellRanger pipeline to align reads, generate feature-barcode matrices. Conclusions: We found that the fate of HE and Non-HE has been decided and separated at the emergence of endothelial precursor population during hPSC hematopoiesis.

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RNA Sequencing Facilitates Quantitative Analysis of Transcriptomes of H1 derived CD31+ cells and H1 after HAND1-overexpression derived CD31+ cells at Day8 after human early hematopoietic differentiation .

Project description:RNA Sequencing Facilitates Quantitative Analysis of Transcriptomes of H1 derived CD31+ cells and H1 after HAND1-overexpression derived CD31+ cells at Day8 after human early hematopoietic differentiation .

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Project description:Purpose:hESCs hematopoietic differentiation recapitulates embryonic hematopoiesis in vivo and occurs through three main stages: mesoderm commitment, hemogenic endothelium (HE) formation and hematopoietic specification. The goal of this study are to increase the understanding of hESCs hematopoietic differentiation process and its regulatory mechanism. Methods: hESC samples were collected through flow cytometry sorting.mRNA profiles of this samples were generated by deep sequencing using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays Conclusions: Compared with other three populations, 57 cell surface markers were highly enriched in CD31+CD34+ endothelial cells.

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Project description:Purpose: The goals of this study are to verify HAND1 overexpression is suffice to upregulate other MAGs in H1 cells during human early hematopoietic differentiation through comparing the transcriptome profilings in WT samples and HAND1-overexpressed samples collected at day 8 after hematopoietic differentiation. Methods: mRNA profiles of hESC samples collected at day 8 after hematopoiesis differentiation were generated by deep sequencing using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays Conclusions: HAND1 overexpression is suffice to upregulate other MAGs during early hematopoietic differentiation of H1 cells.

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RNA Sequencing Facilitates Quantitative Analysis of Transcriptomes of BC1, BC1 derived APLNR+ cells, CD31+CD34+ cells and CD43+ cells during human early hematopoietic differentiation

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RNA Sequencing Facilitates Quantitative Analysis of Transcriptomes of H1, H1 derived APLNR+ cells, CD31+CD34+ cells and CD43+ cells during human early hematopoietic differentiation

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