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Epithelial endoplasmic reticulum stress orchestrates a protective IgA response I

ABSTRACT: Epithelial endoplasmic reticulum stress orchestrates a protective IgA response I

PROVIDER: PRJNA512503 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8383540.fastq.gz	Fastqsanger.gz
	SRR8383541.fastq.gz	Fastqsanger.gz
	SRR8383542.fastq.gz	Fastqsanger.gz
	SRR8383533.fastq.gz	Fastqsanger.gz
	SRR8383543.fastq.gz	Fastqsanger.gz

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Epithelial endoplasmic reticulum stress orchestrates a protective IgA response II

Project description:Epithelial endoplasmic reticulum stress orchestrates a protective IgA response II

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Epithelial endoplasmic reticulum stress orchestrates a protective IgA response I

Project description:Immunoglobulin A (IgA) is the major secretory immunoglobulin isotype at mucosal surfaces where it regulates microbial commensalism and excludes luminal factors from contacting intestinal epithelial cells (IEC). IEC endoplasmic reticulum (ER) stress induces a polyreactive IgA response which protects from small intestinal inflammation. IEC ER stress causes expansion and activation of peritoneal B1b cells independent of microbiota and T cells that culminates in increased lamina propria and luminal IgA. Xbp1dIEC mice exhibit IEC ER stress by conditional deletion of X-box-binding protein 1 (XBP1). Here we examine differentially expressed genes in peritoneal B1b cells of germ-free Xbp1dIEC mice compared to littermate (WT) controls.

2019-02-28 | GSE124561 | GEO

Epithelial endoplasmic reticulum stress orchestrates a protective IgA response II

Project description:Immunoglobulin A (IgA) is the major secretory immunoglobulin isotype at mucosal surfaces where it regulates microbial commensalism and excludes luminal factors from contacting intestinal epithelial cells (IEC). IEC endoplasmic reticulum (ER) stress induces a polyreactive IgA response which protects from small intestinal inflammation. IEC ER stress causes expansion and activation of peritoneal B1b cells independent of microbiota and T cells that culminates in increased lamina propria and luminal IgA. Xbp1dIEC mice exhibit IEC ER stress by conditional deletion of X-box-binding protein 1 (XBP1). Here we examine single-cell transcriptomes of peritoneal cavity cells of germ-free Xbp1dIEC mice (KO) compared to littermate controls (WT).

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Epithelial endoplasmic reticulum stress orchestrates a protective IgA response.

Project description:Immunoglobulin A (IgA) is the major secretory immunoglobulin isotype found at mucosal surfaces, where it regulates microbial commensalism and excludes luminal factors from contacting intestinal epithelial cells (IECs). IgA is induced by both T cell-dependent and -independent (TI) pathways. However, little is known about TI regulation. We report that IEC endoplasmic reticulum (ER) stress induces a polyreactive IgA response, which is protective against enteric inflammation. IEC ER stress causes TI and microbiota-independent expansion and activation of peritoneal B1b cells, which culminates in increased lamina propria and luminal IgA. Increased numbers of IgA-producing plasma cells were observed in healthy humans with defective autophagy, who are known to exhibit IEC ER stress. Upon ER stress, IECs communicate signals to the peritoneum that induce a barrier-protective TI IgA response.

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