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Next Generation Sequencing Facilitates Quantitative Analysis of adipose tissue Treg and LN Treg Transcriptomes

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of adipose tissue Treg and LN Treg Transcriptomes

PROVIDER: PRJNA513260 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8403111_1.fastq.gz	Fastqsanger.gz
	SRR8403111_2.fastq.gz	Fastqsanger.gz
	SRR8403112_1.fastq.gz	Fastqsanger.gz
	SRR8403112_2.fastq.gz	Fastqsanger.gz
	SRR8403113_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing Facilitates Quantitative Analysis of adipose tissue Treg and LN Treg Transcriptomes

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Next Generation Sequencing Facilitates Quantitative Analysis of Lactic acid Treated Human Treg cells Transcriptomes

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Project description:Regulatory T (Treg) cells harbor immune suppressive capacity and are crucial for the maintenance of peripheral tolerance. Treg cells are considered to be heterogenic, where compromised FOXP3 expression results in the generation of exTreg cells. Here we report that the E3 deubiquitinase USP21 prevents the depletion of FOXP3 protein and restricts tissue-resident exTreg cell generation. Mice lacking USP21 in Treg cells display immune disorders characterized by spontaneous T cell activation and excessive T helper type 1 (Th1) skewing. USP21 stabilizes FOXP3 protein by mediating its deubiquitination and therefore helps to maintain the expression of Treg signature genes. Moreover, at inflamed loci, tissue-resident USP21-deficient Treg cells display a Th1-like effector phenotype. Therefore, we demonstrate how USP21 controls the identity of tissue-resident Treg cells by preventing FOXP3 loss.

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