Project description:We performed low input RNA-seq and single-cell RNA-seq to transcriptionally characterize four human innate T cell (ITCs) subsets, namely iNKT, MAIT, Vδ1 expressing γδ T cells, and Vδ2 expressing γδ T cells. We compared ITCs to CD4+ T and CD8+ T cells, representing the adaptive compartment, and to Natural Killer (NK) cells, representing the innate compartment.
Project description:To date, the spatial and functional characterization of human splenic innate lymphoid cells (ILC) has remained limited. Here, the authors integrate CITE-seq and spatial transcriptomic data and present an atlas of human splenic NK and ILC cells, and define high-resolution subset-specific spatial organization and interaction networks, providing insights into the presence of local immune niches in secondary lymphoid organs.
Project description:To date, the spatial and functional characterization of human splenic innate lymphoid cells (ILC) has remained limited. Here, the authors integrate CITE-seq and spatial transcriptomic data and present an atlas of human splenic NK and ILC cells, and define high-resolution subset-specific spatial organization and interaction networks, providing insights into the presence of local immune niches in secondary lymphoid organs.