Project description:To perform miRNA expression analysis through deep sequencing, RNA was isolated from pools of day 1 adult worms treated with enoxacin or vehicle for 12h in M9 medium.
Project description:The goal of this study was to perform transcriptomics on vehicle-, UK5099-, Butyrate-, and UK5099+Butyrate-treated mouse prostate organoids. We used FACS to isolate basal cells from C57BL/6 mouse prostates and treated with small molecules for one week before harvesting for RNA-sequencing.
Project description:RNA sequencing was performed for the DCA- or vehicle-treated ILC2s sorted from the large intestine of C57BL/6 mice (n = 2 per group).
Project description:RNA sequencing was performed for the toyocamycin- or vehicle-treated ILC2s sorted from the large intestine of C57BL/6 mice (n = 3 per group).
Project description:RNA sequencing was performed for the TNF- or vehicle-treated organoids derived from the large intestine of healthy male C57BL/6J mice (n = 3 per group).
Project description:To profile transcriptomic alterations induced by catecholamine stimulation, we treated ApcΔ/Δ, KrasG12D/Δ, Trp53Δ/Δ mouse colon cancer organoids with norepinephrine or vehicle and then performed RNA sequencing.
Project description:Identification of defects in piRNAs and piRNA precursors Sequencing of small RNAs (18-30nt). Sample treatments: Secondary siRNAs in C. elegans carry a 5' triphosphate and this has to be removed prior to cloning, hence the use of 5' polyphosphatase treatment to reveal this population. TAP (tobacco acid pyrophosphatase) is another enzyme that removes 5' triphosphate but it also removes 5' CAP structures- we used this to look for piRNA precursor sequences in C. elegans that have a 5' cap.
Project description:Quantitative proteomics in DIA mode was used to analysis the proteomic profile of 24 weeks from the sorafenib treated and vehicle treated monkeys.