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The gene expression profiles in response to kanamycin antibiotics in Arabidopsis thaliana

ABSTRACT: The gene expression profiles in response to kanamycin antibiotics in Arabidopsis thaliana

PROVIDER: PRJNA516211 | ENA |

REPOSITORIES: ENA

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The gene expression profiles in response to kanamycin antibiotics in Arabidopsis thaliana

Project description:Arabidopsis grown in a plastic pot was used to investigate the kanamycin phytotoxicity to seedling growth. The seedlings was irrigated with solution of different kanamycin concentration. After ten days in treatment, the gene expression profiles was conducted to compare the pattern of antibiotics-induced genes.

2019-01-22 | GSE125396 | GEO

RNA-seq analysis of the effect of kanamycin and the ABC transporter AtWBC19 on Arabidopsis thaliana seedlings reveals changes in metal content

Project description:Plants are exposed to antibiotics produced by soil microorganisms but little is known about their responses at the transcriptional level. Likewise, few endogenous mechanisms of antibiotic resistance have been reported. The Arabidopsis thaliana ATP Binding Cassette (ABC) transporter AtWBC19 (ABCG19) is known to confer kanamycin resistance, but the exact mechanism of resistance is not well understood. Here we examined the transcriptomes of control seedlings and wbc19 mutant seedlings using RNA-seq analysis. Exposure to kanamycin indicated changes in the organization of the photosynthetic apparatus, metabolic fluxes and metal uptake. Elemental analysis showed a 60% and 80% reduction of iron uptake in control and wbc19 mutant seedlings respectively, upon exposure to kanamycin. The drop in iron content was accompanied by the upregulation of the gene encoding for FERRIC REDUCTION OXIDASE 6 (FRO6) in mutant seedlings but not by the differential expression of other transport genes known to be induced by iron deficiency. In addition, wbc19 mutants displayed a distinct expression profile in the absence of kanamycin. Most notably, the expression of several zinc ion binding proteins, including ZINC TRANSPORTER 1 PRECURSOR (ZIP1), was increased, suggesting abnormal zinc uptake. Elemental analysis confirmed a 50% decrease of zinc content in wbc19 mutants. Thus, the antibiotic resistance gene WBC19 appears to also have a role in zinc uptake.

2014-06-20 | GSE58662 | GEO

Comparative transcriptomic response analysis of Escherichia coli K-12 MG1655 to nine antibiotics

Project description:To investigate and compare transcriptomic changes of Escherichia coli K-12 MG1655, the bacterium was exposed to nine antibiotics (tetracycline, mitomycin C ,imipenem, ceftazidime, kanamycin, ciprofloxacin, polymyxin E, erythromycin, and chloramphenicol) , and RNA-Seq was performed to determine comparative transcriptomic changes.

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RNA-seq analysis of the effect of kanamycin and the ABC transporter AtWBC19 on Arabidopsis thaliana seedlings reveals changes in metal content

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Expression profiles of MicroRNA and SiRNA of Arabidopsis thaliana Col-0 and transgenic plants

Project description:Expression profiles of MicroRNA and SiRNA of Arabidopsis thaliana Col-0 and transgenic plants with constitutive expression of the chimeric receptors NRG1 grown at different temperature To reveal the underlying molecular mechanism of de-cosuppression with memory by high temperature in Arabidopsis, we performed the expression profiles of microRNA and SiRNA in transgenic plants with constitutive expression of the chimeric receptors NRG1 and wide type Col-0 grown at different temperature using the Custom LC Sciences Arabidopsis microRNA and SiRNA array. Keywords: high temperature, de-cosuppression, MicroRNA, SiRNA

2008-05-24 | GSE11535 | GEO

Metabolomic profiles in short day, after bolting, single leaf analysis, Arabidopsis thaliana

Project description:Metabolomic profiles in short or long day, before and after bolting, single leaf analysis, Arabidopsis thaliana

2015-09-14 | MTBLS12 | MetaboLights

Metabolomic profiles in long day, after bolting, single leaf analysis, Arabidopsis thaliana

Project description:Metabolomic profiles in short or long day, before and after bolting, single leaf analysis, Arabidopsis thaliana

2015-09-14 | MTBLS7 | MetaboLights

Metabolomic profiles in short day, before bolting, single leaf analysis, Arabidopsis thaliana

Project description:Metabolomic profiles in short or long day, before and after bolting, single leaf analysis, Arabidopsis thaliana

2015-09-14 | MTBLS13 | MetaboLights

Metabolomic profiles in long day, before bolting, single leaf analysis, Arabidopsis thaliana

Project description:Metabolomic profiles in short or long day, before and after bolting, single leaf analysis, Arabidopsis thaliana

2015-09-14 | MTBLS11 | MetaboLights

Transcriptome analysis of Arabidopsis thaliana treated with valinomycin

Project description:The goal of this study is to compare NGS-derived transcriptome profiles of Arabidopsis thaliana with or without valinomycin treatment.

2026-01-26 | GSE316913 | GEO

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