Project description:Thyroid-associated ophthalmopathy (TAO) is a complex eye and orbital disorder that is uniquely linked to Graves’ hyperthyroidism (GH). A popular explanation for the development of orbital inflammation in these patients is autoimmunity against the TSH-receptor expressed in orbital preadipocytes. However, this has not been proven and alternative hypotheses should be entertained. We have shown recently that antibodies against highly purified rabbit skeletal muscle calsequestrin are sensitive and specific markers of eye muscle inflammation in patients with thyroid autoimmunity. Keywords: disease state comparison In the study presented here, RNA from thyroid tissue of hyperthyroid patients with (n=10) and without (n=8) symptoms of ophthalmopathy was compared across 20,589 genes.
Project description:To reveal the potential role of lncRNAs in the extraocular muscles (EOMs) of Graves' ophthalmopathy (GO), we performed lncRNA expression profiling and compared their expression differences between EOM tissues sampled from GO and concomitant esotropia (CET) patients using microarray analysis. To reveal the potential role of lncRNAs in the extraocular muscles (EOMs) of Graves' ophthalmopathy (GO), we performed lncRNA expression profiling and compared their expression differences between EOM tissues sampled from GO and concomitant esotropia (CET) patients using microarray analysis.
Project description:By studying the differences in transcriptional and methylation levels between the GO (Graves' ophthalmopathy) patient group and the GH (Graves' hyperthyroidism) patient group, we aim to explore the pathogenic mechanisms of GO in patients with GH, and provide intervention guidance for the prevention of GO.
Project description:Search for SNPs associated with the pharmacogenomic profile of Benzidazole adverse reactions in Chagas Disease Homo sapiens patients.
Project description:Homo sapiens fresh whole blood was infected with Candida parapsilosis. RNA-pool of both species extracted at 0min (control), 15, 30, 60, 120, 240 min. Samples are rRNA depleted. Measurement of Homo sapiens gene expression.
Project description:To investigate the effects of nimodipine on TGF-β induced fibrotic phenotype switching of human orbital fibroblasts, we performed the RNA sequencing of human orbital fibroblasts (OFs) derived from Graves’ ophthalmopathy (GO) patients and co-cultured with TGF-β for 24 hours,with or without nimodipine pretreatment.
Project description:Homo sapiens fresh whole blood was infected with Candida albicans SC5314. RNA-pool of both species extracted at 0min (control), 15, 30, 60, 120, 240 min. Samples are rRNA depleted. Expression measurement of Homo sapiens genes.
