Project description:Plant propagation requires adventitious root production, and the inability to root cuttings prevents some tree species from being widely used and propagated on a big scale. Studies on the differences in adventitious root production ability between species that are easily rooted and those that are difficult to root are few, despite the fact that many studies have been done to examine the developmental mechanisms of adventitious roots. To attempt to address this problem, we examined the transcriptomes of Populus davidiana (difficult-to-root) and Populus yunnanensis (easy-to-root) spikes at various intervals following cutting. We discovered that the number of differential genes was significantly greater in the 96- and 120-hour groups than in the 0- and 48-hour groups. The majority of growth hormone and cytokinin-related genes were up-regulated in P. yunnanensis, while they were down-regulated in P. davidiana.