Identification of genes are regulated by DROSHA at mRNA level [microarray]
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA517317 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Purpose: DROSHA plays an important role in breast cancer progression. Exploring the gene expression profiles regulated by DROSHA will facilitate to understand the mechanism which is responsible for DROSHA-induced breast cancer development. Results: We found that 105 genes were significantly up-regulated during DROSHA silencing in MDA-MB-231 cells, 200 genes were significantly down-regulated during DROSHA silencing in MDA-MB-231 cells. Conclusions: Our study indicated that DROSHA-mediated 305 differentially expressed genes might be invovled in breast cancer development.
2021-01-28 | GSE125705 | GEO
Project description:Neural stem cell regulation is essential for the formation of the central nervous system and homeostatic neurogenesis in the adult mammalian brain. The RNAseIII Drosha, a key component of the miRNA microprocessor, plays a central role in regulating NSC maintenance partially through a miRNA-independent mechanism. Drosha controls mRNA expression levels by targeting and cleaving evolutionary conserved stem-loop hairpins located in the mRNAs of stem cell-related transcription factors. However, it is unknown how the Drosha-mediated endonucleolytic cleavage of mRNA is regulated. Here, we identify novel Drosha and NFIB interactors in hippocampal NSCs by in vitro pull-down assays followed by Mass Spectrometry. We unravel the RNA binding proteins implicated in Drosha-mediated regulation of neurogenesis and we find Scaffold Attachment Factor B1 to play a novel and essential role in NFIB mRNA regulation during neural stem cell differentiation.
2024-06-03 | PXD017677 | Pride
Project description:Drosha is a type III RNAse, which plays a critical role in miRNA biogenesis. Drosha and its double-stranded RNA-binding partner protein Pasha/DGCR8 likely recognize and cleave miRNA precursor RNAs or pri-miRNA hairpins co-transcriptionally. To identify RNAs processed by Drosha, we used tiling microarrays to examine transcripts after depletion of drosha mRNA with dsRNA in Drosophila Schneider S2 cells. This strategy identified 137 Drosha-regulated RNAs, including 11 putative pri-miRNAs comprising 15 annotated miRNAs. Most of the identified pri-miRNAs seem extremely large, >10 kilobases as revealed by both the Drosha knock down strategy and by RNA PolII chromatin IP followed by Drosophila tiling microarrays. Surprisingly, more than a hundred additional RNAs not annotated as miRNAs are under Drosha control and are likely to be direct targets of Drosha action. This is because many of them encode annotated genes, and unlike bona fide pri-miRNAs, they are not affected by depletion of the miRNA processing factor, dicer-1. Moreover, application of the evofold analysis software indicates that at least 25 of the Drosha-regulated RNAs contain evolutionarily conserved hairpins similar to those recognized by the Drosha-Pasha/DGCR8 complex in pri-miRNAs. One of these hairpins is located in the 5′ UTR of both pasha and mammalian DGCR8. These observations suggest that a negative feedback loop acting on pasha mRNA may regulate the miRNA-biogenesis pathway: i.e., excess Drosha cleaves pasha/DGCR8 primary transcripts and leads to a reduction in pasha/DGCR8 mRNA levels and Pasha/DGCR8 synthesis. Keywords: time course, ChIP-chip
2008-12-25 | GSE14215 | GEO
Project description:CAKUT (congenital anomalies of the kidney and the urinary tract) is one of the most prevalent birth defects, affecting around 1% of newborns. However, the genetic factors underlying the majority of CAKUT are unknown. Drosha, the microRNA-processing ribonuclease, has been reported to regulate the nephric cap meschyme development, and its mutations are implicated in Wilms tumor, a pediatric kidney cancer. Here, we investigated the role of Drosha in mesangial cells and its impact on glomerular capillary tuft formation. Deletion of Drosha in mesangial cells (DroshacKO) disrupted the glomerular capillary tufts formation, leading to dysplastic glomeruli, proteinuria, oliguria, reduced looping of glomerular capillaries and capillary dilation. Drosha-knockdown (KD) in mesangial cells (SV40 MES 13) leads to decreased cell proliferation and reduced Gata3 protein level. Transcriptome analysis by RNA-seq shows decreased level of ribosome protein gene (RPG)s but unaltered mRNA level of major genes affecting kidney development, including Gata3, Pax2, Atn1, Wt1 etc. in Drosha-KD compared to Control SV40 MES13 cells. Further analysis indicates Drosha regulates the translation of Gata3 in mesangial cells via regulating RPG transcription. Our work, for the first time, reveals that Drosha in mesangial cells orchestrates the formation of glomerular capillary tufts by regulating Gata3 translation. It identifies the critical role of DROSHA in nephric development and proposes DROSHA as a novel potential causal gene for CAKUT. [miRNA-Seq] This study investigates the role of DROSHA in microRNA biogenesis using the mouse renal mesangial cell line ME13 sv40. Small RNA sequencing was performed to compare microRNA expression profiles between DROSHA knockdown cells and control cells. The results aim to elucidate how DROSHA depletion alters microRNA expression patterns, which may provide insights into post-transcriptional regulation in renal mesangial cells.
2026-07-10 | GSE302255 | GEO
Project description:Anaysis of mRNA changes in HeLa cells following knockdown of Drosha or DGCR8. Drosha is a nuclear RNase III that carries out microRNA (miRNA) processing by cleaving primary microRNA transcript (pri-miRNA). DGCR8 is an essential co-factor of Drosha. Keywords: gene expression array-based (RNA / in situ oligonucleotide)
2009-02-05 | GSE13639 | GEO
Project description:To determine genes regulated independently of microRNAs in early haematopoietic progenitors (LSKs) we compared the expression profiles of Drosha or Dicer deficient LSKs and control. Those genes differentially expressed between Drosha or Dicer deficient LSKs are likely regulated indepedently of microRNAs as either Drosha or Dicer deletion will lead to a complete and equivalent loss of microRNAs.
2015-09-10 | GSE61305 | GEO
Project description:To determine genes regulated independently of microRNAs in early haematopoietic progenitors (LSKs) we compared the expression profiles of Drosha or Dicer deficient LSKs and control. Those genes differentially expressed between Drosha or Dicer deficient LSKs are likely regulated indepedently of microRNAs as either Drosha or Dicer deletion will lead to a complete and equivalent loss of microRNAs. LSKs were sorted from control, Drosha fl/fl of Dicer fl/fl mice.These cells were activated in vitro for 72 hours to induce total and equivalent deletion of Drosha or Dicer. RNA was extracted after 72 hours.3 repeats of the three groups were analyzed.
2015-09-10 | E-GEOD-61305 | biostudies-arrayexpress
Project description:Identification of SAT1 regulated genes in GBM cells by microarray
| PRJNA478388 | ENA
Project description:Identification of genes are regulated by AURKA at transcription level
| PRJNA527723 | ENA
Project description:We report the ability of the Drosha null/conditional-null mouse model to enable the identification of pri-miRNA transcripts. The conditional-null allele of Drosha phenocopies the null allele both in mESC and in mice, upon conversion to the null state with Cre. Examination of the effects of Drosha deficiency in mouse embryonic stem cells.
2015-02-09 | E-GEOD-55735 | biostudies-arrayexpress