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Metagenomics/metatranscriptomics of Bioelectrochemical reactors

ABSTRACT: Electrode-dependent anaerobic ammonium oxidation by anammox bacteria

PROVIDER: PRJNA517785 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR8556130.fastq.gz	Fastqsanger.gz
	SRR8556131.fastq.gz	Fastqsanger.gz
	SRR8556132_1.fastq.gz	Fastqsanger.gz
	SRR8556132_2.fastq.gz	Fastqsanger.gz

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Transcriptome Kuenenia stuttgartiensis bioreactor

Project description:Anaerobic ammonium-oxidising (anammox) bacteria, members of the ‘Candidatus Brocadiaceae’ family, play an important role in the nitrogen cycle and are estimated to be responsible for about half of the oceanic nitrogen loss to the atmosphere. Anammox bacteria combine ammonium with nitrite and produce dinitrogen gas via the intermediates nitric oxide and hydrazine (anammox reaction) while nitrate is formed as a by-product. These reactions take place in a specialized, membrane-bound compartment called the anammoxosome. Therefore, the substrates ammonium, nitrite and product nitrate have to cross the outer-, cytoplasmic- and anammoxosome membranes to enter or exit the anammoxosome. The genomes of all anammox species harbour multiple copies of ammonium-, nitrite- and nitrate transporter genes. Here we investigated how the distinct genes for ammonium-, nitrite- and nitrate- transport were expressed during substrate limitation in membrane bioreactors. Transcriptome analysis of Kuenenia stuttgartiensis planktonic cells under ammonium-limitation showed that three of the seven ammonium transporter genes and one of the six nitrite transporter genes were significantly upregulated, while another ammonium and nitrite transporter gene were downregulated in nitrite limited growth conditions. The two nitrate transporters were expressed to similar levels in both conditions. In addition, genes encoding enzymes involved in the anammox reaction were differentially expressed, with those using nitrite as a substrate being upregulated under nitrite limited growth and those using ammonium as a substrate being upregulated during ammonium limitation. Taken together, these results give a first insight in the potential role of the multiple nutrient transporters in regulating transport of substrates and products in and out of the compartmentalized anammox cell.

2020-06-05 | GSE148825 | GEO

Anaerobic ammonium oxidation (anammox) reactor metagenome

Project description:Anaerobic ammonium oxidation (anammox) reactor metagenome Metagenome

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Project description:Nitric oxide-dependent anaerobic ammonium oxidation

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anaerobic ammonium oxidation (anammox) bacteria

Project description:anaerobic ammonium oxidation (anammox) bacteria Phenotype or genotype

| PRJNA454513 | ENA

Nitrate-dependent anaerobic propane and butane oxidation coupled to anaerobic ammonium oxidation

Project description:Nitrate-dependent anaerobic propane and butane oxidation coupled to anaerobic ammonium oxidation

2024-05-24 | PXD039266 | Pride

Nitric oxide-dependent anaerobic ammonium oxidation

Project description:Nitric oxide (NO) has several important functions in biology and atmospheric chemistry as a toxin, signaling molecule, ozone depleting agent and the precursor of the greenhouse gas nitrous oxide (N2O). Even though NO is a potent oxidant, and was available on earth earlier than oxygen, its direct use by microorganisms for growth was not demonstrated before. Using physiological experiments, metatranscriptomics and metaproteomics, here we show that anaerobic ammonium-oxidizing (anammox) bacterium Kuenenia stuttgartiensis grow by coupling ammonium oxidation to NO reduction, and produce only N2. Such a metabolism could have existed on early earth, and has implications in controlling N2O and NO emissions both from natural and manmade ecosystems, where anammox bacteria contribute significantly to N2 release to the atmosphere.

2019-02-26 | PXD011763 | Pride

bioreactor metagenome

Project description:anaerobic ammonium oxidation (anammox) reactors Genome sequencing and assembly

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Decoding Microbial Responses to Ammonia Shock Loads in Biogas Reactors through Metagenomics and Metatranscriptomics.

Project description:The presence of elevated ammonia levels is widely recognized as a significant contributor to process inhibition in biogas production, posing a common challenge for biogas plant operators. The present study employed a combination of biochemical, genome-centric metagenomic and metatranscriptomic data to investigate the response of the biogas microbiome to two shock loads induced by single pulses of elevated ammonia concentrations (i.e., 1.5 g NH4+/LR and 5 g NH4+/LR). The analysis revealed a microbial community of high complexity consisting of 364 Metagenome Assembled Genomes (MAGs). The hydrogenotrophic pathway was the primary route for methane production during the entire experiment, confirming its efficiency even at high ammonia concentrations. Additionally, metatranscriptomic analysis uncovered a metabolic shift in the methanogens Methanothrix sp. MA6 and Methanosarcina flavescens MX5, which switched their metabolism from the acetoclastic to the CO2 reduction route during the second shock. Furthermore, multiple genes associated with mechanisms for maintaining osmotic balance in the cell were upregulated, emphasizing the critical role of osmoprotection in the rapid response to the presence of ammonia. Finally, this study offers insights into the transcriptional response of an anaerobic digestion community, specifically focusing on the mechanisms involved in recovering from ammonia-induced stress.

| S-EPMC10785759 | biostudies-literature

Project description:Microbial nitrate-dependent anaerobic propane and butane oxidation coupled to anaerobic ammonium oxidation

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Investigating the autotrophic and mixotrophic metabolism of an anammox bacterium

Project description:Anaerobic ammonium oxidizing (anammox) bacteria mediate a key step in the biogeochemical nitrogen cycle and have been applied worldwide for the energy-efficient removal of nitrogen from wastewater. However, outside their core energy metabolism, little is known about the metabolic networks driving anammox bacterial anabolism and mixotrophy beyond genome predictions. Here, we experimentally resolved the central carbon metabolism using metabolomics (LC-MS and GC-MS), metabolic flux analysis and proteomics (shot-gun proteomics).

2021-07-30 | PXD016171 | Pride

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