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High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes

ABSTRACT: High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes

PROVIDER: PRJNA520989 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8529860_1.fastq.gz	Fastqsanger.gz
	SRR8529860_2.fastq.gz	Fastqsanger.gz
	SRR8529852_1.fastq.gz	Fastqsanger.gz
	SRR8529852_2.fastq.gz	Fastqsanger.gz
	SRR8529853_1.fastq.gz	Fastqsanger.gz

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High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes

Project description:Purpose: Mouse BMDM is the universal cell type of studying innate immunity.This study was to analyze LPS induced innate immune respone and the relationship between serine synthesis pathway and LPS induced innate immune respone Methods: BMDM cells were treated with or without LPS for 6 hours in the pressence of phgdh inhibitor or not. Then mRNA profiles of these samles were generated by High-throughput sequencing analysis, in triplicate, using illumina HiSeqTM 2000. And the differencial mRNA profiles were analyzed. Results: mRNA profiles about 23002 genes were analyzed, and differencial expression profiles were compared . Conclusions: Our study represents the first detailed analysis of transcriptomes of mouse BMDM treated with LPS and phgdh inhibitor, with biologic replicates, generated by RNA-seq technology. This data was useful to analyze the relationship between serine synthesis pathway and LPS induced innate immune respone

2019-09-23 | GSE126084 | GEO

High-throughput sequencing Analysis of Transcriptomes of depolarized mouse BMDM cell

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High-throughput sequencing Analysis of Transcriptomes of depolarized mouse BMDM cell

Project description:Purpose:Mouse BMDM is the universal cell type of studying innate immunity.This study was to analyze LPS induced innate immune respone and the relationship between the membrane potential and LPS induced innate immune respone Methods: WT BMDM cells were treated with or without LPS for 6 hours in the pressence of membranepotential depolarization stimuli. Then mRNA profiles of these samles were generated by High-throughput sequencing analysis, in triplicate, using Illumina HiSeq /Novaseq or MGI2000. And the differencial mRNA profiles were analyzed. Results: mRNA profiles of more than 20000 genes were analyzed, and differencial expression profiles were compared . Conclusions: Our study represents the first detailed analysis of transcriptomes of WT and membrane potential depolarized macrophages, with biologic replicates, generated by RNA-seq technology. This data was useful to analyze the relationship between membrane potential and LPS induced innate immune respone

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High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes after LPS Challenge

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High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes after LPS Challenge

Project description:Purpose: Mouse BMDM is the universal cell type of studying innate immunity.This study was designed to analyze LPS induced innate immune respone and the gene expression in WT and HDAC3 KO BMDMs. Methods: BMDM cells from Hdac3f/f and Lyz2-Cre-Hdac3f/f were treated with or without LPS for 4 hours. Then mRNA profiles of these samles were generated by High-throughput sequencing analysis, in triplicate, using illumina HiSeqTM2000/MiSeq. And the differencial mRNA profiles were analyzed. Results: mRNA profiles were analyzed, and differencial expression profiles were compared . Conclusions: Our study demostrated the transcriptional profiles of BMDMs from Hdac3f/f and Lyz2-Cre-Hdac3f/f mice upon LPS challenge, with biologic replicates, generated by RNA-seq technology.

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