Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of small RNAs interacting with FLAG/HA(FH)-tagged LARP7

ABSTRACT: Identification of small RNAs interacting with FLAG/HA(FH)-tagged LARP7

PROVIDER: PRJNA522460 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8580082.fastq.gz	Fastqsanger.gz
	SRR8580083.fastq.gz	Fastqsanger.gz
	SRR8580084.fastq.gz	Fastqsanger.gz

Items per page:

1 - 3 of 3

Similar Datasets

Identification of small RNAs interacting with FLAG/HA(FH)-tagged LARP7

Project description:The La-related protein LARP7 has been mainly described as a component of the 7SK small nuclear ribonucleoprotein (snRNP) complex, which negatively regulates RNA polymerase II by sequestering the positive transcription elongation factor b (P-TEFb). In our studies, we discovered a novel, 7SK snRNP-independent function of LARP7. We show that LARP7 interacts with the U6 spliceosomal RNA as well as with the small nucleolar RNAs (snoRNAs) directing the 2'-O-methylations of U6. Importantly, in the absence of LARP7, significantly less 2'-O-methylations are deposited on U6 affecting splicing fidelity. Mutations in the LARP7 gene have been associated with the Alazami syndrome, a form of primordial dwarfism characterized by intellectual disability. We describe a novel loss-of-function mutation of LARP7 occurring in Alazami patients and detect reduced 2'-O-methylations of U6 in patient-derived samples. Thus, aberrant posttranscriptional RNA modifications of the spliceosomal U6 snRNA may contribute to the development of this severe disease.

2020-02-03 | GSE126591 | GEO

Genome-wide map of Pol II and Flag-LARP7 in human HeLa cells

Project description:We generated genome-wide maps of Pol II and Flag-LARP7 in human HeLa cells. We find that Flag-LARP7 and Pol II co-locolize on Pol II-specific sn/snoRNA genes.

2017-02-02 | GSE87470 | GEO

FLAG-tagged FOXM1A Plasmid

Project description:FLAG-tagged FOXM1A Plasmid

| PRJEB24606 | ENA

FLAG-tagged hnRNPM iCLIP in AGS cells

Project description:FLAG-tagged hnRNPM iCLIP in AGS cells

| PRJNA737793 | ENA

Identification of small RNAs interacting with LARP7

2020-02-03 | GSE126592 | GEO

Genome-wide maps of chromatin state in HCT116 cells expressing Flag-tagged VCP

Project description:Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for VCP and Flag in HCT116 cells expressing Flag-tagged VCP.

2023-07-18 | GSE201200 | GEO

ChIP-seq of MOZ (Flag-V5-BIO tagged) in mouse mouse craniofacial development

Project description:Carboxy-terminally tagged MOZ (Flag-V5-BIO tagged) was detected by ChIP-seq using anti-V5 antibody (Sigma, A7345) to precipitate chromatin associated with MOZ

2019-07-23 | GSE134607 | GEO

ChIP-seq of C-terminal FLAG-tagged WhiA

Project description:ChIP-seq of C-terminal FLAG-tagged WhiA

| PRJEB104001 | ENA

ChIP-seq of N-terminal FLAG tagged WhiA

Project description:ChIP-seq of N-terminal FLAG tagged WhiA

| PRJEB103860 | ENA

Mapping the genomic occupancy by flag-tagged APOBEC3B protein

Project description:Mapping the genomic occupancy by flag-tagged APOBEC3B protein

| PRJNA795961 | ENA