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A genome-wide CRISPR screen identifies mediators of ferroptosis sensitivity in clear-cell carcinomas

ABSTRACT: A genome-wide CRISPR screen identifies mediators of ferroptosis sensitivity in clear-cell carcinomas

PROVIDER: PRJNA523003 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8587694.fastq.gz	Fastqsanger.gz
	SRR8587695.fastq.gz	Fastqsanger.gz
	SRR8587696.fastq.gz	Fastqsanger.gz
	SRR8587697.fastq.gz	Fastqsanger.gz

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A genome-wide CRISPR screen identifies mediators of ferroptosis sensitivity in clear-cell carcinomas

Project description:Clear-cell carcinomas (CCCs) are a histological group of highly aggressive malignancies commonly originating in the kidney and ovary. CCC tumors are distinguished by aberrant lipid and glycogen accumulation and are inherently refractory to a broad range of anti-cancer therapies. In the study associated with this dataset, we identified an intrinsic vulnerability to ferroptosis associated with the unique metabolic state in CCC cells. However, the mediators of this sensitivity to ferroptosis are unknown. Here we perform a genome-wide CRISPR screen to identify genes that mediate the sensitivity to ML210, a selective inhibitor of glutathione peroxidase 4 (GPX4) and a potent inducer of ferroptotic cell death in 786-O, a clear-cell renal cell carcinoma cell line.

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A genome-wide CRISPR screen identifies mediators of ferroptosis sensitivity in polyunsaturated fatty acid-treated cells

Project description:Polyunsaturated fatty acids (PUFA) sensitize cells to ferroptotic cell death. In the study associated with this dataset, we used UACC257 as a model cell line (intrinsically resistant to ferroptosis) induced to be sensitive to ferroptosis with different PUFAs. We performed genome-wide CRISPR knockout screen to identify genes that mediate the sensitivity to ML210, a selective inhibitor of glutathione peroxidase 4 (GPX4) and a potent inducer of ferroptotic cell death. We identified cytochrome P450 oxidoreductase (POR, CYPOR) as a key player required for ferroptotic cell death among other genes.

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Project description:A genome-wide CRISPR screen identifies mediators of ferroptosis sensitivity in polyunsaturated fatty acid-treated cells

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