Lactuca sativa cultivar:VI185
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA523066 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR8649127_1.fastq.gz | Fastqsanger.gz | |||
| SRR8649127_2.fastq.gz | Fastqsanger.gz | |||
| SRR8649128_1.fastq.gz | Fastqsanger.gz | |||
| SRR8649128_2.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 4 of 4 |
Similar Datasets
Project description:The small RNAs and their targets were characterized in lettuce (Lactuca sativa) genome by deep sequencing the small RNA populations of leaf tissues (cv. Salinas, Cobham and Diana), inoculated with Bremia and mock. The small RNA targets were also validated using PARE/degradome data derived from the same tissues.
2017-04-13 | GSE84280 | GEO
Project description:Small RNAs (21-24 nt) are pivotal regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in diverse eukaryotes, including most if not all plants. MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are the two major types, both of which have a demonstrated and important role in plant development, stress responses and pathogen resistance. In this work, we used a deep sequencing approach (Sequencing-By-Synthesis, or SBS) to develop sequence resources of small RNAs from different Lactuca sativa tissues (including leaves, flowers, and fungus-infected leaves). The high depth of the resulting datasets enabled us to examine in detail critical small RNA features such as size distribution, tissue-specific regulation and sequence conservation between different organs in this species. We also developed database resources and a dedicated website (http://smallrna.udel.edu/) with computational tools for allowing other users to identify new miRNAs or siRNAs involved in specific regulatory pathways, verify the degree of conservation of these sequences in other plant species and map small RNAs on genes or larger regions of the genome under study. Small RNA libraries were derived from leaves and flowers of Lactuca sativa, cultivar Salinas, including leaves infected with the fungus Bremia lactucae, strain CA-III. Total RNA was isolated using TriReagent® (Molecular Research Center) and submitted to Illumina (Hayward, CA, http://www.illumina.com) for small RNA library construction using approaches described in (Lu et al., 2007) with minor modifications. The small RNA libraries were sequenced with the Sequencing-By-Synthesis (SBS) technology by Illumina. PERL scripts were designed to remove the adapter sequences and determine the abundance of each distinct small RNA. We thank Richard Michelmore and Oswaldo Ochoa for providing the plant material as well as Kan Nobuta for assistance with the computational methods.
2011-04-29 | E-GEOD-28322 | biostudies-arrayexpress
Project description:Lactuca sativa cultivar:Cheongchima Genome sequencing
| PRJNA727868 | ENA
Project description:Lactuca sativa cultivar:Salinas Genome sequencing and assembly
| PRJNA173551 | ENA
Project description:Baby leaves of lettuce (Lactuca sativa L.) are widely used to produce ready-to-eat salads all around the year. For this purpose, lettuce plants are cultivated under plastic tunnels and harvested at a very early growth stage, between 30-45 days after sowing depending on the season, cultivar and site of cultivation. Usually, the plastic film covering the cultivation tunnels transmits only a very small amount of the solar UVB radiation and partially attenuates UVA radiation. We used UV treatment post-harvest of fresh-cut lettuce baby leaves to compare the efficiency of two UV emission wavebands centred at 306 and 366 nm (achieved by employing narrow-band UV LEDs sources) to identify putative transcriptomic signatures. The analyses have been carried out supported by and in the frame of Project @CNR 2021, Title "EnhAncement of Natural anTIviral Compounds in ready- to-eat Vegetables", Acronym: ANTI-CoV to Dr. G. Agati. IDP-NAHEP, ICAR, Govt of India to A.R.B.
2025-07-04 | GSE292446 | GEO
Project description:Lettuce is a widely consumed leafy vegetable, valued for its nutritional benefits and culinary versatility. Various breeding initiatives have yielded lettuce varieties such as romaine or cos lettuce (Lactuca sativa var. longifolia), characterized by pigmented germplasm and enhanced tolerance to elevated temperatures. Despite its significance, a notable need exists for targeted study investigations into its chemical composition. Thus, this study aims to define the volatile and non-volatile components of the red romaine Thurinus RZ lettuce cultivar using LC-MS and GC-MS analysis. For LC-MS analysis, a C18 column will be accompanied by gradient elution using (A) water and (B) acetonitrile, each containing 0.1% formic acid as the mobile phase. For GC-MS analysis, the HP-5 column will be accompanied by Helium as the carrier gas. The molecules determined will then be ionized, and fragments will be interpreted and annotated using the mass-to-charge (m/z) ratio, which will further be tabulated.
2025-04-10 | MSV000097600 | MassIVE
Project description:Small RNAs (21-24 nt) are pivotal regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in diverse eukaryotes, including most if not all plants. MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are the two major types, both of which have a demonstrated and important role in plant development, stress responses and pathogen resistance. In this work, we used a deep sequencing approach (Sequencing-By-Synthesis, or SBS) to develop sequence resources of small RNAs from different Lactuca sativa tissues (including leaves, flowers, and fungus-infected leaves). The high depth of the resulting datasets enabled us to examine in detail critical small RNA features such as size distribution, tissue-specific regulation and sequence conservation between different organs in this species. We also developed database resources and a dedicated website (http://smallrna.udel.edu/) with computational tools for allowing other users to identify new miRNAs or siRNAs involved in specific regulatory pathways, verify the degree of conservation of these sequences in other plant species and map small RNAs on genes or larger regions of the genome under study.
2011-04-30 | GSE28322 | GEO
Project description:Lactuca sativa var. crispa cultivar:crispa Genome sequencing
| PRJNA1139985 | ENA
Project description:Lactuca sativa var. crispa cultivar:crispa Genome sequencing
| PRJNA1131287 | ENA
Project description:To understand the role of WRKY10 transcription factor in foliar pigmentation patterns in Marchantia (liverwort) and assess the conservation of its regulatory network in Lactuca sativa var. Redfire(angiosperm).
2024-06-11 | GSE238167 | GEO