Project description:Mu transposon insertions were sequenced in the parents and offspring from 4 maize families using MuSeq2. Male parents were Mu-active while female parents were Mu-inactive, and so all new transposon activity can be associated with the male parent. Matched endosperm and leaf from each offspring were sequenced, allowing inherited insertions to be unambiguously identified as these tissues diverge prior to fertilization (each is derived from a sister sperm cell in pollen during double fertilziation). The data were analyzed to follow the transmission of de novo Mu insertions from parent to offspring.

2025-05-09 | GSE296286 | GEO

Sequencing de novo Mu transposon insertions across maize tissues

Project description:We sequenced insertion sites for the maize Mutator (Mu) transposon in leaf, root, endosperm, and pollen from Mu-active plants and Mu-inactive controls. Inherited insertions were identified using matched tissues, and then the abundance of de novo (post-zygotic) insertions was quantified and converted to variant allele frequencies (VAF). This dataset provides insight into the abundance of new transposon insertions within heterogeneous tissues and factors that shape mutation accumulation in multicellular organisms.

2025-05-09 | GSE279993 | GEO

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